- Clone
- NFL3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neurofilament Light Polypeptide 68kD, Neurofilament Light Polypeptide, Neurofilament Subunit NF-L, CMT1F, NFL, NEFL, NF-L
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![NFL3_A647_Neurofilament_Antibody_1_040518 NFL3_A647_Neurofilament_Antibody_1_040518](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/NFL3_A647_Neurofilament_Antibody_1_040518.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of Alexa Fluor® 647 anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 10X objective. Scale bar: 100 µm -
IHC staining of Alexa Fluor® 647 anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of Alexa Fluor® 647 anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 10X objective. Scale bar: 100 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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845907 | 25 µg | £85 | ||||
845908 | 100 µg | £213 |
Neurofilaments (NF) are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Detection of NF, and the subunits of NF, may serve as a biomarker for axonal degeneration. The degree of axonal degeneration is related to the amount of NF detected in the cerebrospinal fluid (CSF) and the blood. Neurofilament light (NF-L) levels are elevated in Alzheimer’s Disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified macaque NFL.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Product Citations
-
- RRID
-
AB_2728564 (BioLegend Cat. No. 845907)
AB_2734616 (BioLegend Cat. No. 845908)
Antigen Details
- Structure
- Neurofilament light is a 68 kD protein.
- Distribution
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Cytoskeleton and the cytoplasm.
- Function
- Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Zetterberg H, Blennow K. 2015. JAMA Neurol. 11:1237-8.
2. Bäckström D, et al. 2015. JAMA Neurol. 72(10):1175.
3. Janelidze S, et al. 2015. Neurology 85:1834.
4. Magdalinou NK, et al. 2015. J. Neurol. Neurosurg. Psychiatry 86(11):1240.
5. Skillbäck T, et al. 2014. Neurology. 83:1945. - Gene ID
- 4747 View all products for this Gene ID
- UniProt
- View information about Neurofilament L on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Neurofilament L (NF-L) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Neurofilament L (NF-L) | NFL3 | WB,IHC-P |
Alexa Fluor® 594 anti-Neurofilament L (NF-L) | NFL3 | IHC-P |
Alexa Fluor® 647 anti-Neurofilament L (NF-L) | NFL3 | IHC-P |
Alexa Fluor® 488 anti-Neurofilament L (NF-L) | NFL3 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Neurofilament L (NF-L)
Western blot of purified anti-Neurofilament L (NF-L) antibod... IHC staining of purified anti-Neurofilament L (NF-L) antibod... IHC staining of purified anti-Neurofilament L (NF-L) antibod... -
Alexa Fluor® 594 anti-Neurofilament L (NF-L)
IHC staining of Alexa Fluor® 594 anti-Neurofilament L (NF-L)... -
Alexa Fluor® 647 anti-Neurofilament L (NF-L)
IHC staining of Alexa Fluor® 647 anti-Neurofilament L (NF-L)... IHC staining of Alexa Fluor® 647 anti-Neurofilament L (NF-L)... IHC staining of Alexa Fluor® 647 anti-Neurofilament L (NF-L)... -
Alexa Fluor® 488 anti-Neurofilament L (NF-L)
IHC staining of Alexa Fluor® 488 anti-Neurofilament L (NF-L...
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