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Anti-c-Myc Tag (9E10) Affinity Gel

Pricing & Availability
Clone
9E10 (See other available formats)
Regulatory Status
RUO
Other Names
myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E10_c-Myc_Tag_Gel_Purification_121213
Binding capacity of anti-c-Myc agarose for C-terminal c-Myc and internal c-Myc tagged GST.
  • 9E10_c-Myc_Tag_Gel_Purification_121213
    Binding capacity of anti-c-Myc agarose for C-terminal c-Myc and internal c-Myc tagged GST.
  • 9E10_c-Myc_Tag_Gel_Purification_2_121213
    Stable activity of anti-c-Myc agarose over ten rounds of affinity purification.
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658502 500 µL £141
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Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
50% anti-c-Myc-tag (9E10) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3.
Preparation
Affinity purified.
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

Purification of c-Myc tagged fusion protein from bacteria lysates - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Resin binding capacity is greater than 0.5 mg/mL.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Liu L, et al. 2022. Sci Adv. 8:eadd8928. PubMed
  2. Liu L, et al. 2023. Cancers (Basel). 15:. PubMed
  3. Zhang G, et al. 2016. J Virol. 90: 2306 - 2315. PubMed
  4. Shamay-Ramot A, et al. 2015. PLoS Genet. 11: 1005702. PubMed
RRID
AB_3106269 (BioLegend Cat. No. 658502)

Antigen Details

Cell Targets
N-terminal, C-terminal tagged and internal fusion protein
Biology Area
Cell Biology, Protein Purification, Protein Synthesis
Antigen References
  1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
  2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
  3. Battey J, et al. 1983. Cell 34:779.
  4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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