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Anti-CD171 (L1CAM) Antibody (Previously Covance catalog# MMS-172R)

Pricing & Availability
Clone
74-5H7 (See other available formats)
Regulatory Status
RUO
Other Names
Neural cell adhesion molecule L1, antigen identified by monoclonal antibody R1
Previously
Covance Catalog# MMS-172R
Isotype
Mouse IgG1, κ
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Product Citations
publications
74-5H7_ASCITES_CD171_WB_050218
Western blot of Anti-CD171 (L1CAM) antibody (clone 74-5H7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain total lysate. The blot was incubated with 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 74-5H7_ASCITES_CD171_WB_050218
    Western blot of Anti-CD171 (L1CAM) antibody (clone 74-5H7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain total lysate. The blot was incubated with 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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838101 500 µL £297
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Description

L1CAM, also known as CD171, is a neuronal cell adhesion molecule involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against purified chick L1, and binds to the L1 cytoplasmic domain at about T1172 (adjacent to the YRSL sorting signal).
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
Application

WB - Quality tested
ICC, IP, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain. 

Application References

(PubMed link indicates BioLegend citation)
  1. Kamiguchi H, et al. 1998. J. Neurosci. 18(14):5311-21.
  2. Lemmon V, et al. 1989. Neuron. 2(6):1597-603.
  3. Yip PM, Siu CH. 2001. J. Neurochem. 76(5):1552-64.
  4. Schaefer AW, et. al. 2002. J. Cell Biol. 157:1223. (WB, IHC-P, IP)
  5. Beer S, et. al. 1999. J. Cell Sci. 112:2667. (IP)
  6. Itoh K, et. al. 2005. Mol. Cell Neurosci. 29:245. (WB, IP)
  7. Zheng XJ, et. al. 2005. Environ. Technol. 26:1073. (ICC)
  8. Chen SY, et. al. 2001. FASEB. J. 15:1649. (IHC-P) PubMed
  9. Itoh K, et. al. 2004. J. Cell Biol. 165:145. (WB)
  10. Cohen NR, et. al. 1998. Curr. Biol. 8:26. (WB)
Product Citations
  1. Chen S, et al. 2001. FASEB J. 15:1649-1561. PubMed
  2. Hu J, et al. 2022. Cell Death Dis. 13:48. PubMed
  3. Kleene R, et al. 2021. J Neurochem. 157:1102. PubMed
  4. Girbes Minguez M, et al. 2020. FASEB J. 34:9869. PubMed
RRID
AB_2565388 (BioLegend Cat. No. 838101)

Antigen Details

Structure
Expected MW: 200-220 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Gene ID
3897 View all products for this Gene ID
UniProt
View information about CD171 on UniProt.org
Go To Top Version: 3    Revision Date: 09/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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