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Anti-GFP Nanobody Affinity Gel

Pricing & Availability
Clone
LaG-19 (See other available formats)
Regulatory Status
RUO
Other Names
Green fluorescent protein, GFP, tag affinity
Isotype
Llama VH Ig
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Product Citations
publications
LaG-19_Affinity Gel_GFP_08312016
GFP-fused protein was immunoprecipitated from 100 µg transfected 293E cell extract using 10 µl anti-mCherry nanobody Affinity Gel (lane 1, negative control), 10 µl anti-GFP nanobody Affinity Gel (lane 2). Immunoprecipitates and 10% of total input (10 µg, Lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-GFP (clone 1GFP63) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • LaG-19_Affinity Gel_GFP_08312016
    GFP-fused protein was immunoprecipitated from 100 µg transfected 293E cell extract using 10 µl anti-mCherry nanobody Affinity Gel (lane 1, negative control), 10 µl anti-GFP nanobody Affinity Gel (lane 2). Immunoprecipitates and 10% of total input (10 µg, Lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-GFP (clone 1GFP63) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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689302 1 mL £342
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689303 2 mL £562
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689304 5 mL £1203
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Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins. This makes GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Nanobodies are heavy chain only, single-domain antibodies, that lack the presence of light chains. These are typically derived from members of the Camelidae family that include llamas and camels. Their variable region (VHH) is the smallest antigen-binding fragment found in a natural antibody, and nanobodies are the smallest (≈15 kD) naturally occuring immunoglobins. Further, nanobodies are stable, and can bind antigens with high affinity. Experimentally, nanobodies have been applied in WB, IF, and IP. In IP/WB applications, nanobodies can avoid IgG heavy/light chain issues that occur when using regular antibodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Aequorea victoria, Epitope tag
Reported Reactivity
Species independent
Antibody Type
Recombinant
Immunogen
Victoria GFP
Formulation
50% anti-GFP nanobody (clone LaG-19) conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

IP - Quality tested
Recommended Usage

For immunoprecipitation, the suggested use of this reagent is 5 - 20 µl affinity gel for 100 µg lysate harvested from GFP overexpressed cells.

RRID
AB_3105971 (BioLegend Cat. No. 689302)
AB_3105971 (BioLegend Cat. No. 689303)
AB_3105971 (BioLegend Cat. No. 689304)

Antigen Details

Structure
GFP is a 27 kD monomer consisting of 238 amino acids.
Function
Fluorescent protein.
Biology Area
Cell Biology
Antigen References

1. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
2. Chalfie M, et al. 1994. Science 263:802.
3. Cortez-Retamozo V, et al. 2004. Cancer Res. 64:2853.
4. Muyldermans S. 2013. Annu. Rev. Biochem. 82:775.
5. Fridy PC, et al. 2014. Nat. Methods 11:1253.

Gene ID
NA
UniProt
View information about GFP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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