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Anti-Myosin Phosphatase Antibody (Previously Covance catalog# PRB-457C)

Pricing & Availability
Clone
Poly19251 (See other available formats)
Regulatory Status
RUO
Other Names
Protein Phosphatase 1 Regulatory Subunit 12A, Myosin Binding Subunit, Myosin Phosphatase, Target Subunit 1, Protein Phosphatase Myosin-Binding Subunit
Previously
Covance Catalog# PRB-457C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
a-Purified_Myosin_Antibody_WB_032019
Total cell lysates (15 µg total protein) from HepG2, A431 HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-MyPt1 Antibody, clone Poly19251, at a 1:10000 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • a-Purified_Myosin_Antibody_WB_032019
    Total cell lysates (15 µg total protein) from HepG2, A431 HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-MyPt1 Antibody, clone Poly19251, at a 1:10000 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b-Purified_Myosin_Antibody_WB_030515
    Western Blot showing specificity of Myosin Phosphatase. Lane 1: Molecular weight marker; Lane 2: HeLa; Lane 3: BSA neg control.
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925101 100 µL £244
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Description

Myosin phosphatase target subunit 1 (MYPT1) is a regulatory subunit of protein phosphatase 1. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The GTP-bound, active form of RhoA (GTP.RhoA), through its interaction with myosin-binding subunit (MBS) of myosin phosphatase, regulates the phosphorylation of myosin light chain (MLC). Rho inhibits myosin phosphatase through the action of Rho-kinase. Four splice-variant isoforms for the MYPT1 gene have been identified.  

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Dog
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was generated against the N-terminal sequence (residues 1-38) of the large, non-catalytic subunit of myosin phosphatase.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:10000 - 1:20000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

*Predicted MW = 115 kD

The non-catalytic subunit of myosin phosphate, the myosin binding subunit (MBS), is called M130 or MYPT1.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Muranyi A, et al. 1998. Biochem J. 330:225. (WB)
Product Citations
  1. Yu B, et al. 2022. JACC CardioOncol. 4:371. PubMed
RRID
AB_2565464 (BioLegend Cat. No. 925101)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
4659 View all products for this Gene ID
UniProt
View information about Myosin Phosphatase on UniProt.org
Go To Top Version: 5    Revision Date: 04/27/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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