APC anti-β Catenin 1 (CTNNB1) antibody, β Catenin 1, 12F7

Pricing & Availability

Clone: 12F7 (See other available formats)
Regulatory Status: RUO
Other Names: Catenin (Cadherin-Associated Protein), Beta 1, 88 kD, CTNNB1, TNNB, MRD19, armadillo, catenin beta-1
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

SW480 cells (Human colon adenocarcinoma cell line) were treated with True-Nuclear™ Transcription Factor Buffer set and intracellularly stained with anti-β Catenin 1 (CTNNB1) (clone 12F7) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).

Compare all formats See APC spectral data

Cat #

Size

Price

Quantity Check Availability

Save

844607

25 tests

£94

Check Availability

Need larger quantities of this item?
Request Bulk Quote

844608

100 tests

£288

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

β-catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β-catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. Finally, this protein binds to the product of the adenomatous polyposis coli (APC) gene, which is mutated in the adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant β-catenin (N-terminal fragment) fused to MBP.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunfluorescence microscopy⁴ and immunohistochemical staining³.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
RRID: AB_2894517 (BioLegend Cat. No. 844607)
AB_2894517 (BioLegend Cat. No. 844608)

Antigen Details

Structure: 88 kD armadillo repeat containing protein with an unstructured N and C terminal.
Distribution: β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane as well as the cytoplasmic pool of protein. β-catenin can also translocate to the nucleus and is in interphase found in puncta at the proximal end of centrioles. In mitosis, it localizes to the centrosomes at the spindle.
Function: Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition.
Interaction: Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphB-EphrinB signaling.
Biology Area: Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules
Antigen References: 1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
Gene ID: 1499 View all products for this Gene ID
UniProt: View information about beta Catenin 1 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Intracellular Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Other Formats

View All β Catenin 1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-β Catenin 1 (CTNNB1)	12F7	WB,IHC-P,ICFC
Ultra-LEAF™ Purified anti-β Catenin 1 (CTNNB1)	12F7	WB
PE anti-β Catenin 1 (CTNNB1)	12F7	ICFC
APC anti-β Catenin 1 (CTNNB1)	12F7	ICFC

Certificate of Analysis

Customers Also Purchased

Purified anti-Claudin-1

Clone: 9E12C72

Purified anti-β Catenin 1 (CTNNB1)

Clone: 12F7

FITC anti-human CD324 (E-Cadherin)

Clone: 67A4

Recombinant Human L-Selectin (CD62L)-Fc Chimera (carrier-free)

Go To Top Version: 1 Revision Date: 09/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.