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APC anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_APC_080812
HEK-293T cells transfected with plasmid encoding a DYKDDDDK-tagged protein were fixed, permeabilized, and then stained with anti-FLAG-tag (clone L5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • L5_APC_080812
    HEK-293T cells transfected with plasmid encoding a DYKDDDDK-tagged protein were fixed, permeabilized, and then stained with anti-FLAG-tag (clone L5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
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637307 25 µg £78
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637308 100 µg £240
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Schiepers A, et al. 2023. Nature. 615:482. PubMed
  2. Lu V, et al. 2022. Dev Cell. 57:610. PubMed
  3. Draberova H, et al. 2020. EMBO J. 39:e104202. PubMed
  4. Li S, et al. 2022. Elife. 11:. PubMed
  5. Sun Y, et al. 2023. Sci Adv. 9:eade7151. PubMed
  6. Chen L, et al. 2023. Cell Biosci. 13:42. PubMed
  7. Li N, et al. 2023. Nat Commun. 14:1986. PubMed
  8. Feng R, et al. 2020. Antib Ther. 10:3. PubMed
  9. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  10. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  11. Gu T, et al. 2021. Front Immunol. 11:621441. PubMed
  12. Upadhyaya J, et al. 2016. PLoS One. 11: 0156347. PubMed
  13. Halim L, et al. 2022. Front Immunol. 13:836549. PubMed
  14. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  15. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
  16. Liu Y, et al. 2021. Cell. 184:3452. PubMed
  17. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
  18. Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed
  19. Bhatia V, et al. 2022. FASEB Bioadv. 4:574. PubMed
  20. Braun E et al. 2019. Cell Rep. 27(7):2092-2104 . PubMed
  21. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  22. Zah E, et al. 2020. Nat Commun. 11:2283. PubMed
  23. Tong P, et al. 2021. Cell. :. PubMed
  24. Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed
  25. Bosse KR et al. 2017. Cancer cell. 32(3):295-309 . PubMed
  26. Shaik FA, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 316:L45. PubMed
  27. Ahn S, et al. 2019. Cancer Immunol Res. 0.828472222. PubMed
  28. Lu Y, et al. 2020. Immunity. 52:782. PubMed
  29. Uche UU, et al. 2018. J Exp Med. 215:3165. PubMed
  30. Baek MO, et al. 2022. J Cachexia Sarcopenia Muscle. 13:1277. PubMed
  31. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  32. Guo X, et al. 2021. iScience. 24:103295. PubMed
  33. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
  34. Bhagirath AY, et al. 2022. FASEB Bioadv. 4:180. PubMed
  35. Tanigawa Y, et al. 2019. Nat Commun. 10:4064. PubMed
RRID
AB_2561496 (BioLegend Cat. No. 637307)
AB_2561496 (BioLegend Cat. No. 637308)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org
Go To Top Version: 3    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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