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APC anti-human CD114 (G-CSFR) Antibody

Pricing & Availability
Clone
LMM741 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA98
Other Names
CSF3R, HG-CSFR, Colony Stimulating Factor 3 receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
LMM741_APC_021810
Human peripheral blood granulocytes from stained with LMM741 APC
  • LMM741_APC_021810
    Human peripheral blood granulocytes from stained with LMM741 APC
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346107 25 tests £102
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346108 100 tests £228
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Description

CD114 is the receptor of the colony stimulating factor 3 (CSF3, G-CSF), consist of two 130 kD, type I transmembrane chains that form a homodimer. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. CD114 is expressed in all stages of granulocyte differentiation and in monocytes, platelets, endothelial cells, placenta and trophoblasts. The binding of CSF3, results in the activation of many signaling molecules such as Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, and Shp1, resulting in the expression of different target genes that will increase neutrophil precursor survival, proliferation and maturation. In mature neutrophils, it increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CHO cells transfected with hG-CSFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Layton JE, et al. 1997. Growth Factors. 14:117
  2. Layton JE, et al. 1999. J. Biol. Chem. 274:17445
  3. Layton JE, et al. 2001. J. Biol. Chem. 276:36779
  4. Gupta K, et al. 2014. Blood. 123:2550. PubMed
Product Citations
  1. Gupta K, et al. 2014. Blood. 123:2550. PubMed
  2. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
  3. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  4. Bräuer-Hartmann D, et al. 2015. Cancer Res . 75: 3411-3424. PubMed
RRID
AB_2229794 (BioLegend Cat. No. 346107)
AB_2229794 (BioLegend Cat. No. 346108)

Antigen Details

Structure
Single chain type I transmembrane molecule of 130 kD. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. To function as a receptor, this molecules form a homod
Distribution

Granulocytes (in all stages of differentiation), monocytes, platelets, endothelial cells, placenta and trophoblasts.

Function
Stimulates survival, proliferation, and maturation of neutrophil precursors. In mature neutrophils increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).
Interaction
Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, Shp1.
Ligand/Receptor
CSF3(G-CSF)
Bioactivity
Stimulate the proliferation and differentiation of neutrophils precursors and the activation of mature neutrophils.
Cell Type
Endothelial cells, Granulocytes, Monocytes, Neutrophils, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Starnes LM, et al. 2009. Blood 114:1753
2. Skokowa J, et al. 2009. Nat Med. 15:151
3. Ai J, et al. 2008. PLoS One. 3:e3422
4. Germeshausen M, et al. 2008. Curr Opin Hematol. 15:332
5. Marino VJ, Roguin LP, et al. 2008. J Cell Biochem. 103:1512
6. Irandoust MI, et al. 2007. EMBO J. 2:1782

Gene ID
1441 View all products for this Gene ID
UniProt
View information about CD114 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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