APC anti-human CD14 Recombinant Antibody, CD14, QA19A47

Pricing & Availability

Clone: QA19A47 (See other available formats)
Regulatory Status: RUO
Other Names: LPS receptor
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood monocytes were stained with anti-human CD14 recombinant (clone QA19A47) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).

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366805

25 tests

£86

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366806

100 tests

£191

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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone binds to CD14 in human.
RRID: AB_2892446 (BioLegend Cat. No. 366805)
AB_2892446 (BioLegend Cat. No. 366806)

Antigen Details

Structure: Glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein, 53-55 kD
Distribution: Monocytes, macrophages, granulocytes
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor: LPS
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Stocks S, et al. 1990. Biochem J. 268:275.

Wright S, et al. 1990. Science. 249:1434.
Gene ID: 929 View all products for this Gene ID
UniProt: View information about CD14 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD14 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD14 Recombinant Antibody	QA19A47	FC
FITC anti-human CD14 Recombinant Antibody	QA19A47	FC
PE anti-human CD14 Recombinant Antibody	QA19A47	FC
APC anti-human CD14 Recombinant Antibody	QA19A47	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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