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APC anti-human CD158i (KIR2DS4) Antibody

Pricing & Availability
Clone
S22012A (See other available formats)
Regulatory Status
RUO
Other Names
NKAT8, Killer Cell Immunoglobulin-Like Receptor 2DS4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S22012A_APC_CD158i_FC_030425
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) FITC and anti-human CD158i (KIR2DS4) (clone S22012A) APC (left) or mouse IgG1, κ APC isotype control (right).
  • S22012A_APC_CD158i_FC_030425
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) FITC and anti-human CD158i (KIR2DS4) (clone S22012A) APC (left) or mouse IgG1, κ APC isotype control (right).
See APC spectral data
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335105 25 tests £86
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335106 100 tests £250
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins that are expressed on specific subsets of NK and T cells. KIRs are characterized as having either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions, while others with short cytoplasmic region lack ITIMs and have activating functions. Fifteen polymorphic KIR genes have been identified in humans, contributing to the extensive diversity of KIR phenotypes observed in individuals.

CD158i is an activating receptor with two Ig-like domains and a short cytoplasmic region. KIR2DS4 is reported to bind to certain HLA-C molecules and HLA-A*11.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KIR2DS4-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Based on in-house testing on different KIR-expressing transfectants, clone S22012A does not interact with other KIR protein family members.

Antigen Details

Structure
Two Ig-like extracellular domain glycoprotein
Distribution

Subset of NK cells, subset of T Cells

Function
NK cell regulation
Ligand/Receptor
HLA-C, HLA-A*11
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Bottino C, et al. 1996. Eur J immunol. 26:1816-24.
  2. Graef T, et al. 2009. J Exp Med. 206:2557-72.
  3. Campbell KS, et al. 1998. Eur J Immunol. 28:599-609.
Gene ID
3809 View all products for this Gene ID
UniProt
View information about CD158i on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/04/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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