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APC anti-human CD31 Antibody

Pricing & Availability
Clone
WM59 (See other available formats)
Regulatory Status
RUO
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
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Product Citations
publications
WM59_APC_092109
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) APC.
  • WM59_APC_092109
    Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) APC.
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303115 25 tests £78
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303116 100 tests £179
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed
  2. Yousefi M, et al. 2022. Cancer Res. 82:1589. PubMed
  3. Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed
  4. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
  5. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  6. Cortesi EE, et al. 2021. Cells. 10:. PubMed
  7. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  8. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
  9. Junghof J, et al. 2022. NPJ Regen Med. 7:14. PubMed
  10. Jung S, et al. 2015. J Rheumatol. 42:264. PubMed
  11. Wicki A, et al. 2012. Clin Cancer Res. 1.065277778. PubMed
  12. Watanabe Y, et al. 2015. Mol Ther Nucleic Acids. 4: e253. PubMed
  13. Ye W, et al. 2022. Regen Ther. 21:477. PubMed
  14. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  15. Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed
  16. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  17. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
  18. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
  19. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  20. de Sousa BM, et al. 2021. NPJ Regen Med. 6:80. PubMed
  21. Miki K, et al. 2021. Nat Commun. 12:3596. PubMed
  22. Takeda A, et al. 2020. Immunity. 51(3):561-572. PubMed
  23. Singh K, et al. 2017. Mol Ther. 25:2689. PubMed
  24. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
  25. Chu PH, et al. 2020. Chem Res Toxicol. 751:33. PubMed
  26. Bharat A, et al. 2020. Science Translational Medicine. 12(574):. PubMed
  27. Takeda A, et al. 2020. STAR Protoc. 1:100021. PubMed
  28. Antonova LV, et al. 2019. Polymers (Basel). 11:1. PubMed
  29. Wilson SB, et al. 2022. Genome Med. 14:19. PubMed
  30. Liu Z, et al. 2018. Cancer Med. 10.1002/cam4.1584. PubMed
RRID
AB_1877152 (BioLegend Cat. No. 303115)
AB_1877152 (BioLegend Cat. No. 303116)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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