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APC anti-human CD354 (TREM-1) Antibody

Pricing & Availability
Clone
TREM-26 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Triggering receptor expressed on myeloid cells 1, triggering receptor expressed on monocytes 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TREM-26_APC_090310
Human peripheral blood granulocytes stained with TREM-26 APC
  • TREM-26_APC_090310
    Human peripheral blood granulocytes stained with TREM-26 APC
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314909 100 tests £211
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Description

TREM-1 is a 30 kD glycoprotein also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. It is a Type I membrane protein that contains an immunoglobulin-like V-type domain. Alternatively spliced protein variant may be secreted. TREM-1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM-1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM-1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM-1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock. This antibody has been shown to be useful for flow cytometry and activation of monocytes and granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TREM-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Detection: The biotinylated TREM-26 antibody is useful as a detection antibody for a TREM-1 specific sandwich ELISA, when used in conjunction with the TREM-37 antibody (Cat. No. 316102) as the capture antibody. The TREM-26 antibody is useful for flow cytometry and activation of monocytes and granulocytes. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 314913 & 314914).

Application References

(PubMed link indicates BioLegend citation)
  1. Li J, et al. 2011. Dev. Comp. Immunol. epub.
Product Citations
  1. Morris KT, et al. 2014. Br J Cancer. 1211:110. PubMed
RRID
AB_10644181 (BioLegend Cat. No. 314909)

Antigen Details

Structure
Type I membrane protein, 30 kD, contains immunoglobulin-like V-type domain. Alternatively spliced variant may be secreted.
Distribution

Highly expressed in adult liver, lung, and spleen, lower expression in lymph node, placenta, spinal cord, and heart. High expression in peripheral blood myeloid cells.

Function
Stimulates neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines and the upregulation of surface activation markers. Amplifies inflammatory responses to fungal and bacterial
Interaction
DAP12
Modification
Glycosylated
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Bouchon A, et al. 2001. Nature 410:1103.
2. Bouchon A, et al. 2000. J. Immunol. 164:4991.
3. Gingras MC, et al. 2002. Mol. Immunol. 38:817.

Regulation
Upregulated by bacteria, fungi, and LPS
Gene ID
54210 View all products for this Gene ID
UniProt
View information about CD354 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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