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APC anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_AD2_APC_091009
Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).
  • A_AD2_APC_091009
    Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).
  • B_AD2_APC_091009
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
344005 25 tests £114
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344006 100 tests £228
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Liu T, et al. 2020. Biochem Biophys Res Commun. 532:385. PubMed
  2. Yüce M, et al. 2022. J Cell Biochem. 123:1966. PubMed
  3. Gilazieva Z, et al. 2022. Curr Issues Mol Biol. 44:5363. PubMed
  4. Tolcher AW, et al. 2023. J Immunother Cancer. 11: . PubMed
  5. Nushtaeva A, et al. 2023. Int J Mol Sci. 24: . PubMed
  6. Ragni E, et al. 2021. Cells. 10: . PubMed
  7. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  8. Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed
  9. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  10. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  11. Ragni E, et al. 2022. Biomolecules. 12:. PubMed
  12. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  13. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  14. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  15. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  16. Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed
  17. Li J, et al. 2021. Mol Med Rep. 1:23. PubMed
  18. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  19. Hopf A, et al. 2022. Bioengineering (Basel). 9:. PubMed
  20. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
  21. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  22. Bordin A, et al. 2019. Stem Cells Int. 2019:1516746. PubMed
  23. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  24. Moreno-Fernandez M, et al. 2011. Blood. 117:5372. PubMed
  25. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  26. Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed
  27. Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed
  28. Buschmann J, et al. 2013. Cytotherapy. 15:1098. PubMed
  29. Xu L, et al. 2022. Int J Mol Sci. 23:. PubMed
  30. Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed
  31. Siciliano C, et al. 2016. Stem Cell Res. 17: 342-351. PubMed
  32. Safi R, et al. 2021. Photodermatol Photoimmunol Photomed. 37:202. PubMed
  33. Papait A, et al. 2022. Front Immunol. 13:960909. PubMed
  34. Deng P, et al. 2020. Bone Res. 8:39. PubMed
RRID
AB_1877158 (BioLegend Cat. No. 344005)
AB_1877158 (BioLegend Cat. No. 344006)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD73 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F
Purified anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F,Block
PE anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Biotin anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Fire™ 780 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark Red™ 718 anti-hu CD73 (Ecto-5'-nucleotidase) (Flexi-Fluor™) AD2 FC
PerCP/Fire™ 806 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark PLUS B550™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Go To Top Version: 2    Revision Date: 04/09/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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