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APC anti-human Lineage Cocktail (CD3, CD19, CD20, CD56)

Pricing & Availability
Clone
UCHT1; HIB19; 2H7; 5.1H11;
Regulatory Status
RUO
Other Names
Lin1
Isotype
Mouse IgG1, κ/Mouse IgG2b, κ
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Product Citations
publications
APC_Human-Lineage-Cocktail_Antibody_FC_1_101614
Human peripheral blood leukocytes were stained with APC anti-human Lineage Cocktail, HLA-DR Brilliant Violet 421™ and CD123 PE. Top plot is gated on lymphocytes and monocytes and bottom plot is gated on Lineage negative cells.
  • APC_Human-Lineage-Cocktail_Antibody_FC_1_101614
    Human peripheral blood leukocytes were stained with APC anti-human Lineage Cocktail, HLA-DR Brilliant Violet 421™ and CD123 PE. Top plot is gated on lymphocytes and monocytes and bottom plot is gated on Lineage negative cells.
  • APC_Human-Lineage-Cocktail_Antibody_FC_2_101614
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363601 50 tests £401
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Description

This cocktail is optimized for the detection of human peripheral blood T, B, and NK cells. CD3 is found on T, NK, and iNKT cells. CD19 and CD20 are expressed on B cells and CD56 is expressed on NK and NK-T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Bonavia AS, et al. 2022. Front Immunol. 13:1007016. PubMed
  2. Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed
  3. Maheshwari D, et al. 2022. iScience. 25:104384. PubMed
  4. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
RRID
AB_2916117 (BioLegend Cat. No. 363601)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, and neutrophils.

Cell Type
B cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Stem Cells
Molecular Family
CD Molecules
Gene ID
4684 View all products for this Gene ID 915 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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