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APC anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
ACK2 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
A_ACK2_APC_012610
C57BL/6 bone marrow cells stained with ACK2 APC and CD11b (M1/70) FITC
  • A_ACK2_APC_012610
    C57BL/6 bone marrow cells stained with ACK2 APC and CD11b (M1/70) FITC
  • B_ACK2_APC_012610
    C57BL/6 bone marrow cells stained with rat IgG2b APC isotype control and CD11b (M1/70) FITC
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135107 25 µg £57
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135108 100 µg £133
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Description

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine IL-3 dependent mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3

ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshinaga K, et al. 1991. Development 113:689.
  2. Broudy VC, et al. 1996. Blood 88:75.
  3. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.
Product Citations
  1. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  2. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  3. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  4. White Z, et al. 2022. Blood Cells Mol Dis. 97:102688. PubMed
  5. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  6. Arnold I, et al. 2011. J Immunol. 186:6165. PubMed
  7. Hitzler I, et al. 2012. J Immunol. 188:3594. PubMed
  8. Chen Z, et al. 2021. Front Oncol. 11:718731. PubMed
  9. Hutter K, et al. 2020. FEBS J. . PubMed
  10. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  11. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  12. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  13. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  14. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  15. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  16. Rodriguez A, et al. 2021. STAR Protoc. 2:100846. PubMed
  17. Sachs N, et al. 2012. Proc Natl Acad Sci U S A. 109:21468. PubMed
  18. Orlanski S, et al. 2016. Proc Natl Acad Sci U S A. 113: 5018-5023. PubMed
  19. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  20. Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
RRID
AB_2028407 (BioLegend Cat. No. 135107)
AB_2028407 (BioLegend Cat. No. 135108)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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