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APC anti-mouse CD185 (CXCR5) Antibody

Pricing & Availability
Clone
L138D7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, GPCR6, MDR-15
Isotype
Rat IgG2b, κ
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Product Citations
publications
L138D7_APC_CXCR5_Antibody_FC_1_040113
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) APC (top) or rat IgG2b, κ APC isotype control (bottom).
  • L138D7_APC_CXCR5_Antibody_FC_1_040113
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) APC (top) or rat IgG2b, κ APC isotype control (bottom).
  • L138D7_APC_CXCR5_Antibody_FC_2_040113
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145505 25 µg £102
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145506 100 µg £240
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Description

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Product Citations
  1. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  2. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  3. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  4. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  5. Zander R, et al. 2022. Immunity. 55:475. PubMed
  6. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  7. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  8. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  9. Essig K et al. 2017. Immunity. 47(6):1067-1082 . PubMed
  10. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  11. Jing L, et al. 2022. Front Immunol. 13:864995. PubMed
  12. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  13. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  14. Myers DR et al. 2019. Cell reports. 27(6):1858-1874 . PubMed
  15. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  16. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  17. Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
  18. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  19. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  20. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  21. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  22. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  23. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  24. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  25. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  26. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  27. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  28. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
RRID
AB_2561969 (BioLegend Cat. No. 145505)
AB_2561969 (BioLegend Cat. No. 145506)

Antigen Details

Structure
42 kD, seven transmembrane protein
Distribution

B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain

Function
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor
BLC/CXCL13
Cell Type
B cells, T cells, Tfh
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.

Gene ID
12145 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/05/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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