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APC anti-mouse CD24 Antibody

Pricing & Availability
Clone
30-F1 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Stable Antigen (HSA), HsAg, Ly-52, Nectadrin
Isotype
Rat IgG2c, κ
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Product Citations
publications
30-F1_APC_CD24_Antibody_FC_053115
C57BL/6 mouse splenocytes were stained with CD24 (clone 30-F1) APC (filled histogram) or rat IgG2c, κ APC isotype control (open histogram).
  • 30-F1_APC_CD24_Antibody_FC_053115
    C57BL/6 mouse splenocytes were stained with CD24 (clone 30-F1) APC (filled histogram) or rat IgG2c, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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138505 25 µg £78
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138506 100 µg £240
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Description

CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, hematopoietic stem cells, and neurons. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus/spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (FC)
Product Citations
  1. Xia KS, et al. 2023. Bioact Mater. 21:69. PubMed
  2. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  3. Feng H, et al. 2020. J Clin Invest. 130:6354. PubMed
  4. Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
  5. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  6. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  7. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  8. Jiang G, et al. 2021. Cell Death Dis. 12:979. PubMed
  9. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  10. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  11. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  12. Shen Y, et al. 2022. Cell Death Dis. 13:749. PubMed
  13. Mead BE et al. 2018. BMC biology. 16(1):62 . PubMed
  14. Ying Z, et al. 2020. Cell Stem Cell. 26(3):403-419. PubMed
  15. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_2565650 (BioLegend Cat. No. 138505)
AB_2565650 (BioLegend Cat. No. 138506)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD.
Distribution

Lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, and neurons

Function
Regulate B and T cell proliferation and differentiation.
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Lymphocytes, Monocytes, Neurons, Thymocytes
Biology Area
Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Li YS, et al. 1993. J. Exp. Med. 178:951.
2. Aigner S, et al. 1995. Int. Immunol. 7:1557.
3. Hunte BE, et al. 1998. Eur. J. Immunol.. 28:3850.
4. Auerbach R, et al. 1996. Stem Cells 14:269.
5. Allman DM, et al. 1993. J. Immunol.. 151:4431.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org
Go To Top Version: 1    Revision Date: 06/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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