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APC anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEC13dot3_APC_072808.jpg
C57BL/6 splenocytes stained with MEC13.3 APC
  • MEC13dot3_APC_072808.jpg
    C57BL/6 splenocytes stained with MEC13.3 APC
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102509 25 µg £64
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102510 100 µg £194
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang L, et al. 2018. Bone Res. 6:22. PubMed
  2. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  3. J Gautam, X Zhang, Y Yao 2016. Sci Rep. 6:36450. PubMed
  4. Kataru RP, et al. 2021. Sci Signal. 14:. PubMed
  5. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  6. Tao X, et al. 2022. EMBO J. 41:e110439. PubMed
  7. Hahner F, et al. 2022. Redox Biol. 55:102381. PubMed
  8. Cattaneo P, et al. 2022. Nat Commun. 13:7444. PubMed
  9. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  10. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  11. Shao X, et al. 2023. Cell Discov. 9:44. PubMed
  12. Concepcion CP, et al. 2022. Cancer Discov. 12:562. PubMed
  13. Almada AE, et al. 2021. Cell Reports. 34(4):108656. PubMed
  14. Cayre S, et al. 2020. Development. 147:00:00. PubMed
  15. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
  16. Ahmed M, et al. 2016. Development. 143: 1663 - 1673. PubMed
  17. Del Priore I, et al. 2021. STAR Protocols. 2(2):100583. PubMed
  18. Wang M, et al. 2022. Theranostics. 12:4513. PubMed
  19. Watanabe C, et al. 2022. Life (Basel). 12:. PubMed
  20. Chiche A, et al. 2016. Oncogene. 10.1038/onc.2016.396. PubMed
  21. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  22. Bajwa A, et al. 2014. J Am Soc Nephrol. 26:908. PubMed
  23. Wosczyna MN, et al. 2021. Cell Stem Cell. 28(7):1323-1334.e8. PubMed
  24. Romagnoli M, et al. 2020. Development. 147:. PubMed
  25. Wang X, et al. 2022. Nat Aging. 2:155. PubMed
  26. Khateb M, et al. 2022. Cell Rep. 40:111219. PubMed
  27. Xu Q, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  28. Romagnoli M, et al. 2019. Stem Cell Reports. 1.077083333. PubMed
  29. Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed
  30. Bresson L, et al. 2018. Development. 145. PubMed
  31. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  32. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  33. Gao X, et al. 2013. J Exp Med. 210:2833. PubMed
  34. LaFave LM, et al. 2020. Cancer Cell. 38(2):212-228.e13. PubMed
  35. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  36. Auvinen K, et al. 2019. Sci Rep. 9:15698. PubMed
  37. Umezawa Y, et al. 2017. J Biol Chem. 292:19639. PubMed
  38. Rantakari P, et al. 2016. Nature. 538:392-396. PubMed
  39. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  40. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  41. Durré T, et al. 2018. Nat Commun. 9:5178. PubMed
  42. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  43. Jäger M, et al. 2018. Antioxid Redox Signal. 30. PubMed
  44. Mueller A, et al. 2016. Nature. 540:276-279. PubMed
  45. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  46. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  47. He H, et al. 2021. JCI Insight. 6:. PubMed
  48. Ishikura S, et al. 2021. J Cell Biochem. . PubMed
  49. Wenzel J, et al. 2020. Arterioscler Thromb Vasc Biol. 40:2837. PubMed
  50. Peuhu E, et al. 2017. EMBO J. 36(2):165-182. PubMed
  51. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  52. Elias S, et al. 2015. PLoS Biol. 13:1002142. PubMed
  53. Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed
  54. Bruno C, et al. 2020. Brain Commun. 2:fcaa133. PubMed
  55. Liu L et al. 2018. Cell stem cell. 23(4):544-556 . PubMed
  56. Adachi K et al. 2018. Cell stem cell. 23(2):266-275 . PubMed
  57. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  58. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  59. Bozoglu T, et al. 2022. Adv Sci (Weinh). 9:e2103867. PubMed
  60. Cayre S, et al. 2020. Development. . PubMed
  61. Sarshad AA, et al. 2018. Mol Cell. 71:1040. PubMed
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RRID
AB_312916 (BioLegend Cat. No. 102509)
AB_312916 (BioLegend Cat. No. 102510)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 2    Revision Date: 07/19/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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