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APC anti-mouse CD34 Antibody

Pricing & Availability
Clone
HM34 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosialin
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM34_APC_CD34_Antibody_071217
Mouse fibroblast NIH/3T3 cells were stained with CD34 (Clone HM34) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
  • HM34_APC_CD34_Antibody_071217
    Mouse fibroblast NIH/3T3 cells were stained with CD34 (Clone HM34) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
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128611 25 µg £78
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128612 100 µg £203
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Description

CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CD34 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.

Product Citations
  1. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  2. Wang MX, et al. 2022. FASEB J. 36:e22562. PubMed
  3. Li X, et al. 2022. Cell. 185:1709. PubMed
  4. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  5. Wang Y, et al. 2023. J Nanobiotechnology. 21:109. PubMed
  6. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  7. Dusseault J, et al. 2016. Diabetes. 65: 2652 - 2666. PubMed
  8. Yin M et al. 2019. Cell Rep. 27(9):2709-2724 . PubMed
  9. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  10. Huang Z, et al. 2020. Mol Med Rep. 1.979166667. PubMed
  11. Park M, et al. 2021. BMB Rep. 54:470. PubMed
  12. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  13. Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed
  14. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  15. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  16. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  17. Wara AK, et al. 2020. Cell Rep. 33:108550. PubMed
  18. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  19. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  20. Eislmayr K, et al. 2022. Sci Adv. 8:eabj7293. PubMed
  21. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  22. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  23. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  24. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  25. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
RRID
AB_10553895 (BioLegend Cat. No. 128611)
AB_10553895 (BioLegend Cat. No. 128612)

Antigen Details

Structure
Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells

Function
Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, and others.
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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