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APC anti-mouse CD370 (CLEC9A, DNGR1) Antibody

Pricing & Availability
Clone
7H11 (See other available formats)
Regulatory Status
RUO
Other Names
DNGR-1, C-type lectin domain family 9 member A, CD370
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
7H11_APC_CD370_FC_1_092415
C57BL/6 mouse splenocytes were stained with CD11c PE, CD8α PerCP, CD45R/B220 FITC, CD4 FITC, and CD370 (CLEC9A, clone 7H11) APC (top) or rat IgG1, κ APC isotype control (bottom). Data shown was gated on CD8α+, CD45R/ B220-, and CD4- cells.
  • 7H11_APC_CD370_FC_1_092415
    C57BL/6 mouse splenocytes were stained with CD11c PE, CD8α PerCP, CD45R/B220 FITC, CD4 FITC, and CD370 (CLEC9A, clone 7H11) APC (top) or rat IgG1, κ APC isotype control (bottom). Data shown was gated on CD8α+, CD45R/ B220-, and CD4- cells.
  • 7H11_APC_CD370_FC_2_092415
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143505 25 µg £94
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143506 100 µg £240
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Description

CD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Sancho D, et al. 2008. J. Clin. Invest. 118:2098. (IF, IP, WB)
  2. Joffre OP, et al. 2010. Eur. J. Immunol. 40:1255. (ELISA)
RRID
AB_2566379 (BioLegend Cat. No. 143505)
AB_2566379 (BioLegend Cat. No. 143506)

Antigen Details

Structure
Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
Distribution

Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells

Function
Cross present antigen to CTL
Interaction
Necrotic cells
Ligand/Receptor
Ubiquitous preformed acid-labile protein associated ligand(s)
Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens
Antigen References

Poulin LF, et al. 2012. Blood 119:6052.

Gene ID
232414 View all products for this Gene ID
UniProt
View information about CD370 on UniProt.org
Go To Top Version: 1    Revision Date: 10/14/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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