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APC anti-mouse CD93 (AA4.1, early B lineage) Antibody

Pricing & Availability
Clone
AA4.1 (See other available formats)
Regulatory Status
RUO
Other Names
AA4.1, C1qRp, Early B lineage antigen
Isotype
Rat IgG2b, κ
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Product Citations
publications
AA4.1_APC_1_060310
Balb/c bone marrow cells stained with CD45R/B220 (RA3-6B2) PE and AA4.1 APC
  • AA4.1_APC_1_060310
    Balb/c bone marrow cells stained with CD45R/B220 (RA3-6B2) PE and AA4.1 APC
  • AA4.1_APC_2_060310
    Balb/c bone marrow cells stained with CD45R/B220 (RA3-6B2) PE and rat IgG2b, κ APC isotype control
Compare all formats See APC spectral data
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136509 25 µg £62
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136510 100 µg £195
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Description

CD93 is a 130-140kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen. It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. CD93 expression level on splenic immature/transitional B cells is much lower than in bone marrow. It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. It is also expressed on monocytes, macrophages, and endothelial cells. Macrophages from CD93 (-/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo, indicating CD93 may contribute to the in vivo clearance of dying cells. Binding of CD93 to C1q remains controversial.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Pre-B lymphoma 70Z/3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. McKearn JP, et al. 1984. J. Immunol. 132:332
  2. Hanihara-Tatsuzaawa F, et al. 2014. J Biol Chem. 289:30925. PubMed
Product Citations
  1. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  2. Takano J, et al. 2022. Nat Commun. 13:7159. PubMed
  3. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  4. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  5. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  6. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  7. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  8. Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed
  9. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  10. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  11. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  12. Uslu K, et al. 2014. J Immunol. 192:3582. PubMed
  13. Hutter K, et al. 2020. FEBS J. . PubMed
  14. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  15. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  16. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  17. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  18. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  19. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  20. Fiala G, et al. 2015. J Exp Med. 212: 1693-1708. PubMed
  21. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  22. Walker JS, et al. 2021. Nat Commun. 12:6338. PubMed
  23. Roberto MP, et al. 2021. Immunity. 54(8):1807-1824.e14. PubMed
  24. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  25. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID
AB_2275868 (BioLegend Cat. No. 136509)
AB_2275868 (BioLegend Cat. No. 136510)

Antigen Details

Structure
A 130-140 kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen.
Distribution
Expressed during immature B lymphocytes, hematopoietic progenitors and stem cells in bone marrow, immature dendritic cells, monocytes, macrophages, and endothelial cells.
Function
CD93 plays an important role in maintaining of plasma cells in bone marrow niches. It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages.
Cell Type
B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Steinberger P, et al. 2002. J. Leukoc. Biol. 71:133
2. Chevrier S, et al. 2009. Proc. Nat. Acad. Sci. U. S. A. 106:3895
3. Norsworthy PJ, et al. 2004. J. Immunol. 172:3406
4. Li YS, et al. 1996. Immunity 5:527
5. Szilvassy SJ, et al. 1993. Blood 81:2310

Gene ID
17064 View all products for this Gene ID
UniProt
View information about CD93 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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