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APC anti-mouse DR3 (TNFRSF25) Antibody

Pricing & Availability
Clone
4C12 (See other available formats)
Regulatory Status
RUO
Other Names
Apo-3, LARD, TNFR25, TRAMP, WSL-1, DR3
Isotype
Armenian Hamster IgG
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Product Citations
publications
4C12_APC_DR3_Antibody_1_052317
Mouse splenocytes were stained with CD4 FITC and anti-mouse DR3 (clone 4C12) APC (top) or Armenian Hamster IgG, APC isotype control (bottom).
  • 4C12_APC_DR3_Antibody_1_052317
    Mouse splenocytes were stained with CD4 FITC and anti-mouse DR3 (clone 4C12) APC (top) or Armenian Hamster IgG, APC isotype control (bottom).
  • 4C12_APC_DR3_Antibody_2_052317
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144407 25 µg £111
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Description

DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mtnfr25-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

4C12 is an agonistic antibody. It leads to rapid and selective proliferation of pre-existing Tregs in vivo through TCR engagement with MHC class II (MHC-II) and IL-2 signaling. We recommend our Ultra-LEAF™ purified 4C12 antibody (Cat. No. 144411 and 144412) for agonist or in vivo studies.

Application References

(PubMed link indicates BioLegend citation)
  1. Fang L, et al. 2008. J. Exp. Med. 205:1037.
  2. Wolf D, et al. 2012. Transplantation. 94:569. PubMed
  3. Schreiber TH, et al. 2012. J. Immunol. 189:3311.
  4. Longman RS, et al. 2014. J Exp Med. 211:1571. PubMed
RRID
AB_2687247 (BioLegend Cat. No. 144407)

Antigen Details

Structure
55 kD glycoprotein, contains one death domain and four TNFR-Cys repeats
Distribution

CD4, CD8, NKT and Tregs at low level

Function
Plays a role in lymphocyte homeostasis, induces NFKB activation and apoptosis
Ligand/Receptor
TL1-A
Cell Type
NKT cells, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Taraban VY, et al. 2011. J. Clin. Invest. 121:463.
  2. Slebioda TJ, et al. 2011. Eur. J. Immunol. 41:2606.
  3. Schreiber TH, et al. 2010. J. Clin. Invest. 120:3629.
  4. Reddy PB, et al. 2012. J. Virol. 2012. [Epub].
Gene ID
85030 View all products for this Gene ID
UniProt
View information about DR3 on UniProt.org
Go To Top Version: 0    Revision Date: 05/25/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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