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APC anti-mouse LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW7-16B4 (See other available formats)
Regulatory Status
RUO
Other Names
Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TW7-16B4_APC_1_062911
CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC and LAP (clone TW7-16B4) APC (top) or mouse IgG1, κ APC isotype control (bottom), then intracellularly stained with FOXP3 PE. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_APC_1_062911
    CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC and LAP (clone TW7-16B4) APC (top) or mouse IgG1, κ APC isotype control (bottom), then intracellularly stained with FOXP3 PE. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_APC_2_062911
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
141405 25 µg £94
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141406 100 µg £240
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Description

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Tgfb1-transduced P3U1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.

Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1. 

Application References

(PubMed link indicates BioLegend citation)
  1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
  2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations
  1. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  2. Vick SC, et al. 2021. J Immunol. 207:2598. PubMed
  3. Gerlach BD, et al. 2021. Cell Metab. 33:2445. PubMed
  4. Suresh V, et al. 2022. FASEB J. 36:e22449. PubMed
  5. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  6. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  7. Yao G, et al. 2023. Nat Commun. 14:3604. PubMed
  8. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  9. Kumar SRP, et al. 2020. Front Immunol. 11:844. PubMed
  10. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  11. Tan X, et al. 2021. Front Oncol. 11:768222. PubMed
  12. Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed
  13. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  14. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  15. Mukhopadhyay D, et al. 2015. PLoS Negl Trop Dis. 9: e0004145. PubMed
  16. Umeshappa CS, et al. 2021. Cell Reports. 34(13):108919. PubMed
  17. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  18. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  19. Cai B, et al. 2020. Cell Metabolism. 31(2):406-421.e7.. PubMed
  20. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
RRID
AB_10898159 (BioLegend Cat. No. 141405)
AB_10898159 (BioLegend Cat. No. 141406)

Antigen Details

Structure
Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution

Many cell types, highly expressed on activated Tregs and platelets

Function
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor
TGF-β receptors
Cell Type
Dendritic cells, Platelets, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org
Go To Top Version: 2    Revision Date: 08/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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