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APC anti-mouse/rat CD42d Antibody

Pricing & Availability
Clone
1C2 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
1C2_APC_CD42d_Antibody_FC_121514
C57BL/6 platelets (resting) were stained with CD42d (clone 1C2) APC (filled histogram) or Armenian Hamster IgG APC istoype control (open histogram).
  • 1C2_APC_CD42d_Antibody_FC_121514
    C57BL/6 platelets (resting) were stained with CD42d (clone 1C2) APC (filled histogram) or Armenian Hamster IgG APC istoype control (open histogram).
Compare all formats See APC spectral data
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148505 25 µg £86
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148506 100 µg £223
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Description

CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
  2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
  3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
Product Citations
  1. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  2. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  3. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  4. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
RRID
AB_2564601 (BioLegend Cat. No. 148505)
AB_2564601 (BioLegend Cat. No. 148506)

Antigen Details

Structure
83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).
Distribution
Resting platelets and megakaryocytes.
Function
CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.
Ligand/Receptor
CD42d.
Cell Type
Platelets, Megakaryocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sivaraman B, et al. 2011. Biomaterials. 32:5365.
2. Berger G, et al. 1996. Blood. 87:1385.
3. Ravanat C, et al. 1997. Blood. 89:3253.
4. Andrews RK, et al. 1998. Biochemistry. 37:638.
5. Inoue O, et al. 2008. 283:16279.
6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327.
7. Shimomura T, et al. 1990. Blood. 75:2349.
8. Wu G, et al. 1997. Blood. 90:2660.

Gene ID
14729 View all products for this Gene ID
UniProt
View information about CD42d on UniProt.org
Go To Top Version: 1    Revision Date: 12/17/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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