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APC anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_APC_031808
C57BL/6 mouse splenocytes stained with H57-597 APC
  • H57-597_APC_031808
    C57BL/6 mouse splenocytes stained with H57-597 APC
Compare all formats See APC spectral data
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109211 25 µg £53
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109212 100 µg £136
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  3. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  4. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  5. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  6. Zindl CL, et al. 2022. Immunity. 55:494. PubMed
  7. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  8. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
  9. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  10. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  11. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  12. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  13. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  14. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  15. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  16. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  17. Andrews D, et al. 2008. J Virol. 82:4931. PubMed
  18. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  19. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  20. Chen L, et al. 2018. Immunology. 155:123. PubMed
  21. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  22. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  23. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
  24. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  25. Mahadevan NR, et al. 2021. Cancer Discov. 11:1952. PubMed
  26. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  27. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  28. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  29. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  30. Mallis R, et al. 2015. Proc Natl Acad Sci U S A. 112: 8373 - 8378. PubMed
  31. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  32. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  33. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  34. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  35. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  36. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  37. Chuang YT, et al. 2019. J Allergy Clin Immunol. 143:565. PubMed
  38. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  39. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  40. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  41. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  42. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  43. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  44. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  45. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  46. Zhu M, et al. 2012. J Biol Chem. 287:8135. PubMed
  47. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  48. Kanuma T, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1600731. PubMed
  49. Zhang B, et al. 2021. Nature. 599:471. PubMed
  50. Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed
  51. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  52. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  53. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  54. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  55. Preece R, et al. 2020. Mol Ther Methods Clin Dev. 0.895138889. PubMed
  56. Andrews D, et al. 2010. J Exp Med. 207:1333. PubMed
  57. Ley S 2009. J Immunol. 183:865. PubMed
  58. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
RRID
AB_313434 (BioLegend Cat. No. 109211)
AB_313434 (BioLegend Cat. No. 109212)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 2    Revision Date: 02/06/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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