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APC anti-mouse TCR Vγ3 Antibody

Pricing & Availability
Clone
536 (See other available formats)
Regulatory Status
RUO
Other Names
Vγ3 T-cell receptor, T cell receptor gamma 3, TCR Vγ5 (Tonegawa nomenclature), TRGV5, GV1S1
Isotype
Syrian Hamster IgG
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Product Citations
publications
536_APC_062911
Mouse dendritic epidermal cell line 7-17 were stained with anti-mouse TCR Vγ3 (clone 536) APC (filled histogram) or hamster IgG APC isotype control (open histogram).
  • 536_APC_062911
    Mouse dendritic epidermal cell line 7-17 were stained with anti-mouse TCR Vγ3 (clone 536) APC (filled histogram) or hamster IgG APC isotype control (open histogram).
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137505 25 µg £102
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137506 100 µg £232
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Description

Vγ3+ T cells are the predominant γδ TCR-bearing cells in early fetal thymus. The majority of CD3+ cells in 14-day fetal thymus express Vγ3. Vγ3 is also expressed on Thy-1+ dendritic epidermal cells (Thy-1+ DECs). Cells that express Vγ3 are not significantly detectable in adult lymphoid organs. It has been reported that Lck and Fyn, two Src family kinases, are required for the development of Vγ3+ T cells.

Clone 536 reacts with the Vγ3 segment as designated by the Garman nomenclature system. Mouse TCR Vγ3 is also known as TCR Vγ5 under the Heilig & Tonegawa nomenclature system.

Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
AKR mouse dendritic epidermal cell clone 7-17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for relevant formats) application include: immunoprecipitation1 and immunofluorescence2.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. This availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Havran WL, et al. 1989. P. Natl. Acad. Sci. USA 86:4189. (IP)
  2. Havran WL, et al. 1999. J. Immunol. 142:1422. (IF)
  3. Uchida Y, et al. 2011. J. Immunol. 186:6945. PubMed
  4. Hu S, et al. 2013. J. Immunol. 190:3267. PubMed.
Product Citations
  1. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  2. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  3. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
RRID
AB_10895900 (BioLegend Cat. No. 137505)
AB_10895900 (BioLegend Cat. No. 137506)

Antigen Details

Structure
A member of Ig superfamily, heterodimerized with δ TCR chain and associated with CD3 complex.
Distribution

Dendritic epidermal T cells (DECs) and fetal murine thymocytes.

Function
Antigen recognition
Ligand/Receptor
Antigens
Cell Type
T cells, Thymocytes
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679.
2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.
3. Kelly KA, et al. 1993. Int Immunol. 5:331.
4. Moore TA, et al. 1996. J. Immunol. 157:2366.
5. Payer E, et al. 1991. J. Immunol. 146:2536.
6. Van Oers NSC, et al. 1996. Immunity 5:429.

Gene ID
110067 View all products for this Gene ID
UniProt
View information about TCR Vgamma3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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