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APC anti-mouse/rat TNF-α Antibody

Pricing & Availability
Clone
TN3-19.12 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
Isotype
Armenian Hamster IgG
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Product Citations
publications
TN3-19dot12_APC_TNF-alpha_Antibody_1_112817
C57BL/6 splenocytes were stimulated with Cell Activation Cocktail (with Brefeldin A, Cat# 423303 ) for 6 hours, fixed, permeabilized (Fixation Buffer Cat# 420801, Permeabilization Wash Buffer Cat# 421002), and then stained with TNF-α (clone TN3-19.12) APC (top) or Armenian Hamster IgG APC (bottom).
  • TN3-19dot12_APC_TNF-alpha_Antibody_1_112817
    C57BL/6 splenocytes were stimulated with Cell Activation Cocktail (with Brefeldin A, Cat# 423303 ) for 6 hours, fixed, permeabilized (Fixation Buffer Cat# 420801, Permeabilization Wash Buffer Cat# 421002), and then stained with TNF-α (clone TN3-19.12) APC (top) or Armenian Hamster IgG APC (bottom).
  • TN3-19dot12_APC_TNF-alpha_Antibody_2_112817
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506107 25 µg £70
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506108 100 µg £195
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-α (TNF-α) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Reported Reactivity
Rabbit
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1: The Ultra-LEAF™ Purified TN3-19.12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated Poly5160 antibody (Cat. No. 516003) as the detecting antibody for detecting mouse/rat TNF-a. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Clone TN3-19.12 has been shown to neutralize murine TNF-a and cross reacts wtih mouse TFN-ß1. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Aside free, 0.2 µm filtered) is recommended for neutralization (Cat. Nos. 506109 - 506114).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan, K., et al. 1989. J. Immunol. 142:3884. (Neut)
  2. Merrick, B.A., et al. 1992. Appl. Theor. Electrophor 2:177.
  3. Takahashi, S., et al. 1995. Brain Res 690:241.
  4. Rabinovich, R., et al. 1993. J. Pharmacol. Exp. Ther. 267:1550.
  5. Molloy, R., et al. 1993. J. Immunol. 151:2142.
  6. Finkelman, F., et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  7. Simkins, HM., et al. 2011. J. Leukoc Biol. 89:753. PubMed.
  8. Faas MM, et al. 2003. J. Reprod. Immunol. 1:39-52.
Product Citations
  1. He Y, et al. 2022. Adv Healthc Mater. 11:e2200905. PubMed
  2. Komegae EN, et al. 2019. Front Immunol. 10:1496. PubMed
  3. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  4. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  5. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_2721314 (BioLegend Cat. No. 506107)
AB_2721314 (BioLegend Cat. No. 506108)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 18-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID
21926 View all products for this Gene ID 24835 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Other Formats

View All TNF-α Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse / rat TNF-α TN3-19.12 ICFC
Purified anti-mouse / rat TNF-α TN3-19.12 ELISA Capture,IP,WB
APC anti-mouse/rat TNF-α TN3-19.12 ICFC
Ultra-LEAF™ Purified anti-mouse/rat TNF-α TN3-19.12 ELISA Capture,ELISPOT Capture,ICFC,IP,Neut,WB
Go To Top Version: 0    Revision Date: 11/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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