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APC anti-rat CD200 Antibody

Pricing & Availability
Clone
OX-2 (See other available formats)
Regulatory Status
RUO
Other Names
OX-2, OX2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-2_APC_CD200_Antibody_031821
LOU rat splenocytes were stained with anti-rat RT1D FITC and anti-rat CD200 (clone OX-2) APC (left) or mouse IgG1, κ APC isotype control (right).
  • OX-2_APC_CD200_Antibody_031821
    LOU rat splenocytes were stained with anti-rat RT1D FITC and anti-rat CD200 (clone OX-2) APC (left) or mouse IgG1, κ APC isotype control (right).
See APC spectral data
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204809 100 µg £146
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Description

CD200, known as OX-2, is a type I membrane glycoprotein member of the Ig supergene family. CD200 is expressed on B cells, a subset of T cells, thymocytes, follicular dendritic cells, neurons, keratinocytes, vascular endothelium, and some smooth muscle. The interaction of CD200 with CD200 receptor provide a potent costimulatory T-cell signal in the presence of TCR signaling, stimulate macrophages, and inhibit mast cell degranulation. It was reported that increased expression of OX-2 on DC was associated with inhibition of cytokine production and renal allograft rejection. Incubation of lymphocytes with OX-2 Fc inhibits a primary mixed lymphocyte reaction in vitro, decreased IL-2 and IFN-γ production, increased IL-4 and IL-10 production. In vivo infusion of OX-2 Fc promotes both skin and renal graft survival and decreases the antibody response. The OX-2 antibody reacts with rat OX-2 antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

1. Dick, A.D., et al. 2001. Invest Ophthalmol. Vis. Sci. 42:170.

RRID
AB_2890731 (BioLegend Cat. No. 204809)

Antigen Details

Structure
A 47kD (thymus) or 41-kD (brain) type I membrane glycoprotein member of the Ig supergene family. CD200 molecule contains two extracellular Ig-like domains, an Ig V-like, and an Ig C-like domain.
Distribution

CD200 is expressed on B cells, a subset of T cells, thymocytes, follicular dendritic cells, neurons, vascular endothelium, and some smooth muscle.

Function
The interaction of CD200 with CD200 receptor provide a potent costimulatory T-cell signal in the presence of TCR signaling, stimulate macrophages, and inhibit mast cell degranulation.
Ligand/Receptor
CD200 receptor
Cell Type
B cells, Dendritic cells, Endothelial cells, Neurons, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. McMaster WR, et al. 1979. Eur. J. Immunol. 9:426
2. Barclay A. N, et al. 1981. Immunology. 44:727
3. Bukovsky A, et al. 1984. Immunol. 52:631
4. Borriello F, et al. 1997. J. Immunol. 158:4548
5. Gorczynski R. M, et al. 1999. J. Immunol. 163:1654
6. Gorczynski L, et al. 1999. J. Immunol. 162:774

Gene ID
24560 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org
Go To Top Version: 1    Revision Date: 03/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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