APC/Cyanine7 anti-human CD257 (BAFF, BLYS) Antibody, CD257, 1D6

Pricing & Availability

Clone: 1D6 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)
Isotype: Mouse IgG1, κ
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Product Citations: publications

1D6_CD257_APC_CYANINE7_Antibody_091319 — Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with anti-human CD257 (clone 1D6) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram).

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366511

25 tests

£126

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366512

100 tests

£262

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Description

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD257 transfected murine cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2814320 (BioLegend Cat. No. 366511)
AB_2814320 (BioLegend Cat. No. 366512)

Antigen Details

Structure: Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.
Distribution: Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA/ionomycin treatment.
Function: Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Ligand/Receptor: BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR/BR3).
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family: CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References: 1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Moore PA, et al. 1999. Science 285:260.
3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680.
4. Yu G, et al. 2000. Nat. Immunol. 1:252.
5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.
Gene ID: 10673 View all products for this Gene ID
UniProt: View information about CD257 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

BAFF in B-Cell Signaling

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Other Formats

View All CD257 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD257 (BAFF, BLYS)	1D6	FC,IHC-P
Biotin anti-human CD257 (BAFF, BLYS)	1D6	FC
PE anti-human CD257 (BAFF, BLYS)	1D6	FC
APC anti-human CD257 (BAFF, BLYS)	1D6	FC
APC/Cyanine7 anti-human CD257 (BAFF, BLYS)	1D6	FC
TotalSeq™-C0863 anti-human CD257 (BAFF, BLYS)	1D6	PG
TotalSeq™-B0863 anti-human CD257 (BAFF, BLYS)	1D6	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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