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APC/Fire™ 750 anti-GATA3 Antibody

Pricing & Availability
Clone
W19195B (See other available formats)
Regulatory Status
RUO
Other Names
GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W19195B_APC-FIRE-750_GATA3_ICFC-1_122723
Murine Th2 cell line D10.G4.1 was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) APC/Fire™ 750 (filled histogram), or rat IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • A. W19195B_APC-FIRE-750_GATA3_ICFC-1_122723
    Murine Th2 cell line D10.G4.1 was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) APC/Fire™ 750 (filled histogram), or rat IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • B. W19195B_APC-FIRE-750_GATA3_ICFC-2_122723
    Th2 polarized human peripheral blood lymphocytes were surface stained with anti-human CD3 (clone UCHT1) PE. Cells were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) APC/Fire™ 750 or rat IgG2a, κ APC/Fire™ 750 isotype control (right).
Compare all formats See APC/Fire™ 750 spectral data
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386905 25 tests £126
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386906 100 tests £294
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Description

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines. This is responsible for the preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defects in GATA3 result in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human GATA3 recombinant protein (1-258 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone W19195B does not block 16E10A23, TWAJ, or L50-823.

RRID
AB_3097419 (BioLegend Cat. No. 386905)
AB_3097419 (BioLegend Cat. No. 386906)

Antigen Details

Structure
48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus
Distribution

Nucleus

Function
GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T) GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation
Interaction
Interacts with Smad3, FOG1, FOG2, and PU.1
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Yagi R, et al. 2011. Int Immunol. 23:415-20.
  2. Chou J, et al. 2010. J Cell Physiol. 222:42-9.
  3. Ho IC, et al. 2009. Nat Rev Immunol. 9:125-35.
  4. Rothenberg EV, et al. 2008. Semin Immunol. 20:236-46.
  5. Kouros-Mehr H, et al. 2008. Curr Opin Cell Biol. 20:164-70.
  6. Van Esch H, et al. 2000. Nature. 406:419-22.
Gene ID
2625 View all products for this Gene ID
UniProt
View information about GATA3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/27/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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