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APC/Fire™ 750 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Allophycocyanin, SAv-APC
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Product Citations
publications
Strepavidin_APCFIRE_Antibody_FC_110816
Human peripheral blood lymphocytes were stained with biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-APC/Fire™ 750.
  • Strepavidin_APCFIRE_Antibody_FC_110816
    Human peripheral blood lymphocytes were stained with biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-APC/Fire™ 750.
See APC/Fire™ 750 spectral data
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405250 100 µg £141
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Fire™ 750 is useful for detecting biotinylated antibodies. The excitation of APC/Fire™ 750 by 600-635 nm laser light induces a fluorescence emission maximum of 787 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin APC/Fire™ 750 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin APC/Fire™ 750 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Fire™ 750 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

*APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Streptavidin-Allophycocyanin/Fire™ 750 (APC/Fire™ 750 ) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Fire™ 750 is 350 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Maresz K, et al. 2005. J. Neurochemistry 95:437.
  2. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.PubMed.
  3. Ponomarev ED, et al. 2007. J. Immunol. 178:39.
  4. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed
  5. Ponomarev ED,et al.2005.J. Immunol Methods.300:32. PubMed
  6. Cao YA, et al. 2008. Blood PubMed
  7. Garidou L, et al. 2009. J. Virol. PubMed
  8. Dolgachev V, et al. 2009. J. Immunol. 183:5705. PubMed
  9. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed.
  10. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed
  11. Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed
  12. Prince J, et al. 2013. J. Immunol. 191:4709. PubMed
  13. Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed
  14. Orre M, et al. 2014. Neurbiol Aging. 35:1. PubMed
  15. Barbera M, et al. 2014. Gut. 64:11. PubMed
  16. Zhang Y, et al. 2014. Infect Immun. 82:1698. PubMed
  17. Peterson BC, et al. 2014. J Leukoc Biol. 95:809. PubMed
  18. Barbera M, et al. 2015. Gut. 64:11. PubMed
  19. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
Product Citations
  1. Chen GD, et al. 2023. Sci Adv. 9:eabo7605. PubMed
  2. Mudd PA, et al. 2022. Cell. 185:603. PubMed

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Go To Top Version: 2    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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