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APC Syrian Hamster IgG Isotype Ctrl Antibody

Pricing & Availability
Clone
SHG-1 (See other available formats)
Regulatory Status
RUO
Isotype
Syrian Hamster IgG
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Product Citations
publications
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402012 100 µg £146
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Description

The SHG-1 immunoglobulin is of Syrian hamster IgG isotype and was chosen as an isotype control after screening on variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Excitation Laser
Red Laser (633 nm)
Application Notes

The SHG-1 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays as negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood. 106:4241.
Product Citations
  1. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  2. Sastourné-Arrey Q, et al. 2023. Nat Commun. 14:80. PubMed
  3. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  4. Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed
  5. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  6. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  7. Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
RRID
AB_2814701 (BioLegend Cat. No. 402012)

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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