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APC/Fire™ 750 anti-mouse/human CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
DECMA-1 (See other available formats)
Regulatory Status
RUO
Other Names
E-Cadherin, Cadherin-1, CDH1, and UVO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
DECMA-1_APCFire750_CD324_Antibody_090418
MDCK epithelial cell line was stained with CD324 (clone DECMA-1) APC/Fire™ 750 (filled histogram) or rat IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • DECMA-1_APCFire750_CD324_Antibody_090418
    MDCK epithelial cell line was stained with CD324 (clone DECMA-1) APC/Fire™ 750 (filled histogram) or rat IgG1, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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147313 25 µg £117
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147314 100 µg £257
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Description

CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cynomolgus, Dog, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E-Cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, biological function1,2, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
  2. Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
  3. Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
RRID
AB_2750301 (BioLegend Cat. No. 147313)
AB_2750301 (BioLegend Cat. No. 147314)

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor
αEβ7 integrin.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.

Gene ID
999 View all products for this Gene ID 12550 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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