AZDye 647 Recombinant Human CCL5

Pricing & Availability
Regulatory Status
RUO
Other Names
SIS-delta, T-cell specific protein p288, Regulated on Activation Normal T-expressed and Secreted (RANTES), Chemokine (C-C Motif) Ligand 5, TCP228, C-C Motif Chemokine Ligand 5
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Human_AZDye647_CCL5_RECOM_1_092624
Human peripheral blood lymphocytes were surface stained with Alexa Fluor® 647 rat IgG2b, κ isotype control (Cat. No. 400626) (left panel), Alexa Fluor® 647 anti-human CD195 (CCR5) (Cat. No. 359114) (middle panel), or with AZDye 647 Recombinant Human CCL5 (right panel).
  • Human_AZDye647_CCL5_RECOM_1_092624
    Human peripheral blood lymphocytes were surface stained with Alexa Fluor® 647 rat IgG2b, κ isotype control (Cat. No. 400626) (left panel), Alexa Fluor® 647 anti-human CD195 (CCR5) (Cat. No. 359114) (middle panel), or with AZDye 647 Recombinant Human CCL5 (right panel).
  • Human_AZDye647_CCL5_RECOM_2_072924
    Biological activity was performed in HEK293T cells expressing human CCR5 by measuring its ability to recruit β-arrestin2. The ED50 for this effect was calculated at 8 to 30 ng/mL.
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598612 10 µg £257
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Description

CCL5, also known as RANTES, is a CC chemokine that directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5 expression has been associated with a wide range of inflammatory disorders and pathologies. It has been shown that CCL5 is highly expressed by breast tumor cells at primary tumor sites and is strongly associated with the progression of breast cancer. CCL5 may contribute to tumor progression by recruiting monocytes into tumor sites, and CCL5 can also stimulate the release of MMP, thereby promoting tumor cell migration. Some studies also show that CCL5 has pro-angiogenesis effects in vivo and in vitro. CCL5 oligomerization and binding to GAGs can induce angiogenic effects through its receptor CCR1 and CCR5. CCL5 is expressed in the early stages of many viral infections. It has been shown that CCL5 is protective against many viruses including influenza, RSV, HCMV, HCV, and HIV. CCL5 can inhibit HIV infection by competing with the virus for CCR5 binding. CCL5 also has been implicated in the development of asthma, atherosclerosis, and fibrosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CCL5, amino acids Ser24-Ser91 (Accession # P13501) with a C-terminal AZDye647, was expressed in E. coli.
Molecular Mass
The 68 amino acid recombinant protein (Ser24-Ser91) has a predicted molecular mass of 7.8 kDa.
Purity
> 95%, as determined by mass spectrometry
Formulation
Lyophilized from 0.2 µm filtered solution of protein in DI-water.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
Lyophilized, 10 µg per vial
Storage & Handling
Lyophilized at -20°C to -80°C until expiration
Lyophilized at 4°C for 6 months
Lyophilized at Room Temperature 1 month
Reconstituted at -20°C to -80°C for 12 months
Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg/mL up to 1 mg/mL in 0.22 µm filtered DI-water. Avoid repeated freeze/thaw cycles.
Activity
Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR5. The ED50 for this effect was measured at 8 to 30 ng/mL.
Application

FC, Live cell imaging

Application Notes

Protein has been verified for bioactivity prior to conjugation

Antigen Details

Structure
CCL5 is a 68 amino acids molecule with a predicted molecular weight of 7.8 kDa. PDB ID – 1B3A
Distribution

CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. CCL5 is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C

Function
CCL5 can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. CCL5 modulates LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation occurs through the induction of IL-10 by CCL5.
Interaction
CCL5 is a chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils
Ligand/Receptor
CCL5 binds to CCR1, CCR3, and CCR5 receptors. CCL5 also binds to CCR9 but does not induce calcium flux, and therefore there is no chemotactic response.
Bioactivity
Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR5. The ED50 for this effect was measured at 8 to 30 ng/mL.
Cell Type
Basophils, Dendritic cells, Eosinophils, Leukocytes, Lymphocytes, Macrophages, Mast cells, Monocytes, NK cells, NKT cells, T cells
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines, Innate Immune Signaling
Antigen References
  1. Schall TJ, et al. 1990. Nature. 347:669-71.
  2. Cocchi F, et al. 1995. Science. 270:1811-5.
  3. Shahrara S, et al. 2006. J Immunol. 177:5077-87.
  4. Larrubia JR, et al. 2008. World J Gastroenterol. 14:7149-59.
  5. Gleissner CA, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1920-7.
  6. Marques RE, et al. 2013. Expert Opin Ther Targets. 17:1439-60.
Gene ID
P13501 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org
Go To Top Version: 1    Revision Date: 07/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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