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Biotin anti-β-Amyloid, 1-42 Antibody (Previously Covance catalog# SIG-39144)

Pricing & Availability
Clone
12F4 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9144-02
Signet Catalog# 9144-05
Signet Catalog# 9144-10
Covance Catalog# SIG-39144
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_12F4_Biotin_bAmyloid_1-42_IHCP_060618
IHC staining of Biotin anti-β-Amyloid, 1-42 antibody (clone 12F4) on formalin-fixed paraffin-embedded human Alzheimer’s disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slides were counterstained with hematoxylin and bluing solution. The images were captured using a 40X objective. Scale bar: 50 µm
  • A_12F4_Biotin_bAmyloid_1-42_IHCP_060618
    IHC staining of Biotin anti-β-Amyloid, 1-42 antibody (clone 12F4) on formalin-fixed paraffin-embedded human Alzheimer’s disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slides were counterstained with hematoxylin and bluing solution. The images were captured using a 40X objective. Scale bar: 50 µm
  • B_12F4_Biotin_bAmyloid_1-42_ELISA_060618
    Direct ELISA of Biotin anti-β-Amyloid, 1-42 antibody (clone 12F4) binding to plate-immobilized human and rodent Aβ1-42, human Aβ1-40 peptides and recombinant human APP751 protein. ELISA was performed by coating wells with 100 ng of peptides or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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805504 200 µL £407
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Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Do not store antibody diluted below 50 µg/mL in the absence of protein (i.e.: add 2% of bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.25 – 1.0 µg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.01 – 1.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC-P) and Direct ELISA. 

This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 42nd amino acid.

Additional reported applications (for the relevant formats) includes: immunohistochemical staining of paraformaldehyde-fixed frozen sections6 and ELISA3,4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Helwig M, et al. 2013. J. Biol. Chem. 288:1114. (IHC-P)
  2. Takami M, et al.2009. J. Neurosci.; 29: 13042. (IHC-P)
  3. Cenini G, et al. 2012. Biochim Biophys Acta. 1822(2):130. (ELISA) PubMed
  4. Sundaram RK, et al. 2012. Int. J. Pept. Res. Ther. 18(2):99. (ELISA) PubMed
  5. Savage MJ, et al. 2014. J. Neurosci. 8:2884. (ELISA) PubMed
  6. Mastrangelo MA, Bowers WJ. 2008. BMC Neurosci. 9:81. (IHC-F) PubMed
Product Citations
  1. Zhu Q, et al. 2022. Med Phys. 49:1357. PubMed
  2. Cenini G, et al. 2012. Biochim Biophys Acta. 1822:130-138. PubMed
  3. Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed
  4. Gutknecht MF, et al. 2022. Cell Rep Methods. 2:100214. PubMed
  5. Wu Y, et al. 2019. Nat Commun. 10:58. PubMed
  6. Sundaram R, et al. 2012. Int J Pept Res Ther. 18:99-106. PubMed
RRID
AB_2564688 (BioLegend Cat. No. 805504)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle. 
Distribution: cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-42 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 4    Revision Date: 06/07/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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