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Biotin anti-Ezrin Antibody

Pricing & Availability
Clone
CPTC-Ezrin-1 (See other available formats)
Regulatory Status
RUO
Other Names
Cytovillin, Cytovillin 2, CVIL, CVL, Villin-2, VIL2, p81, HEL-S-105
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
CPTC-Ezrin-1_Biotin_Ezrin_Antibody_121919.png
IHC staining of Biotin anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_Biotin_Ezrin_Antibody_121919.png
    IHC staining of Biotin anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_Biotin_Ezrin_Antibody_2_121919.png
    IHC staining of Biotin anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_Biotin_Ezrin_Antibody_3_121919.png
    IHC staining of Biotin anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
866405 25 µg £89
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866406 100 µg £206
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Description

Ezrin, a member of the ERM (Ezrin-Radixin-Moesin) family, is a linker protein between cytoskeleton and cellular membrane. It has wide temporal and spatial expression, and participates in a variety of cellular processes such as cell adhesion, cell motility, cell-cell communication, and apoptosis. High levels of Ezrin expression promotes tumorigenesis in tissues, including glioblastoma, breast cancer, and colon cancer. Ezrin is also highly expressed in neural stem cells and neuroblasts, where it may function in actin remodeling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human Ezrin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820166 (BioLegend Cat. No. 866405)
AB_2820166 (BioLegend Cat. No. 866406)

Antigen Details

Structure
Human Ezrin is a 586 amino acid protein with a molecular mass of 70 kD.
Distribution

Tissue distribution: Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve
Cellular distribution: Cytoskeleton and plasma membrane

Function
Ezrin is involved in the connection of major cytoskeletal structures to the plasma membrane.
Interaction
Interacts with many proteins, such as MPP5, SLC9A3R2, MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B
Cell Type
Astrocytes, Leukocytes, Neural Stem Cells, Neurons, Oligodendrocytes, T cells
Biology Area
Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Cytoskeletal Proteins, Scaffold Proteins
Antigen References
  1. Moon Y, et al. 2013. Histochem. Cell. Biol. 139:403-13.
  2. Xie JJ, et al. 2011. J. Histochem. Cytochem. 59(11):1001-8.
Gene ID
7430 View all products for this Gene ID
UniProt
View information about Ezrin on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Ezrin Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Ezrin CPTC-Ezrin-1 WB,IHC-P
Biotin anti-Ezrin CPTC-Ezrin-1 IHC-P
Go To Top Version: 1    Revision Date: 12/19/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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