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Biotin anti-human CD235ab Recombinant Antibody

Pricing & Availability
Clone
QA20B11 (See other available formats)
Regulatory Status
RUO
Other Names
Glycophorin A/B, GPA/GPB
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20B11_BIOTIN_CD235ab_Antibody_1_072822
Human peripheral blood erythrocytes were stained with biotinylated anti-human CD235ab recombinant (clone QA20B11) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram) followed by PE Streptavidin.
  • QA20B11_BIOTIN_CD235ab_Antibody_1_072822
    Human peripheral blood erythrocytes were stained with biotinylated anti-human CD235ab recombinant (clone QA20B11) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram) followed by PE Streptavidin.
  • QA20B11_BIOTIN_CD235ab_Antibody_2_072822
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X (10 mM, pH 6.0) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/mL biotinylated anti-human CD235ab recombinant (clone QA20B11) (left) or biotinylated mouse IgG1, κ isotype control (right) at 4°C overnight. The next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 Streptavidin (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • QA20B11_BIOTIN_CD235ab_Antibody_3_093022
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X (10 mM, pH 6.0) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/mL biotinylated anti-human CD235ab recombinant (clone QA20B11) (left) or biotinylated mouse IgG1, κ isotype control (right) and purified anti-human IgD (clone W18340A) at 4°C overnight. The next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 Streptavidin (red) and Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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382004 100 µg £137
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Description

The clone QA20B11 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is a major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The clone QA20B11 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.004 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927849 (BioLegend Cat. No. 382004)

Antigen Details

Structure
Sialoglycoprotein, 20 kD
Distribution

Erythrocytes

Cell Type
Erythrocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235ab on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 07/28/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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