Biotin anti-human CD8a Antibody anti-CD8a

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Clone: C8/144B (See other available formats)
Regulatory Status: RUO
Other Names: T8, Leu2
Isotype: Mouse IgG1, κ
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Product Citations: publications

C8slash144B_Biotin_CD8a_Antibody_080118 — Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD8a (clone C8/144B) Biotin at 4°C overnight, followed by 2.5 µg/ml of streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.

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372908

100 µg

£129

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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of the alpha chain of the human CD8 molecule.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include activation³, flow cytometry⁴, immunohistochemical staining of frozen tissue sections², and Western blotting⁵.
RRID: AB_2750103 (BioLegend Cat. No. 372908)

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8β.
Distribution: Majority of thymocytes, T cell subset, NK cells.
Function: MHC class I co-receptor, thymic differentiation, T-cell activation.
Ligand/Receptor: MHC class I molecules.
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88.
Gene ID: 100387674 View all products for this Gene ID
UniProt: View information about CD8a on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD8a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD8a	C8/144B	IHC-P,Activ,FC,IHC-F,WB
Alexa Fluor® 594 anti-human CD8a	C8/144B	IHC-P,SB
Alexa Fluor® 647 anti-human CD8a	C8/144B	IHC-P,ICFC,3D IHC,SB
Biotin anti-human CD8a	C8/144B	IHC-P
Spark YG™ 570 anti-human CD8a	C8/144B	IHC-P
TotalSeq™-Bn1320 anti-human CD8a	C8/144B	SB

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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