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Biotin anti-human IFN-α2 Antibody

Pricing & Availability
Clone
W21083E (See other available formats)
Regulatory Status
RUO
Other Names
IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B
Isotype
Mouse IgG2b, κ
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Product Citations
publications
W21083E_BIOTIN_IFNa2_061724
Direct ELISA showing biotin anti-human IFN-α2 (clone W21083E) bound to plate-immobilized recombinant human IFN-α2 protein (Cat. Nos. 592702, 592704, 592706, and 592708). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W21083E_BIOTIN_IFNa2_061724
    Direct ELISA showing biotin anti-human IFN-α2 (clone W21083E) bound to plate-immobilized recombinant human IFN-α2 protein (Cat. Nos. 592702, 592704, 592706, and 592708). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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636253 50 µg £339
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Description

Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. They are also highly conserved among  mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). IFN-α2 also induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α has been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α. Therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant IFN-α2 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W21083E) could function as a detection antibody when paired with purified anti-human IFN-α2 (Cat No. 632252) (clone W20057G) as the capture antibody and recombinant human IFN-α2 protein (Cat. Nos. 592702, 592704, 592706, and 592708) as the assay standard.

RRID
AB_3662351 (BioLegend Cat. No. 636253)

Antigen Details

Distribution

IFN-α is ubiquitously expressed

Function
IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2 induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
Interaction
Varieties of cells express the IFNα receptor (IFNAR)
Ligand/Receptor
IFNAR complex has two components, IFNAR1 and IFNAR2
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Bose A and Baral R. 2007. Immunol. Lett. 108:68-77.
  2. Zhao W, et al. 2008. J. Immunol. 180:5483-9.
  3. Badiger R, et al. 2012. Plos One. 7:e46779.
  4. Aghemo A, et al 2010. Nat Rev Gastroenterol Hepatol. 7:485-94.
  5. Becker-Merok A, et al. 2013. Lupus. 22:155-63.
  6. Mukherjee KK, et al. 2012. Indian J Med. Res. 136:54-9.
Gene ID
3440 View all products for this Gene ID
UniProt
View information about IFN-α2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 06/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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