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Biotin anti-human IL-1β Antibody

Pricing & Availability
Clone
JK1B-2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Human_IL-1beta_TMB_020405
  • Human_IL-1beta_TMB_020405
Cat # Size Price Quantity Check Availability Save
508301 50 µg £81
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508302 500 µg £286
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-2 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-2 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-2 antibody can neutralize the bioactivity of natural or recombinant IL-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-1 beta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-1β protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection: The biotinylated JK1B-2 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JK1B-1 antibody (Cat. No. 508202/508204) as the capture antibody.

Product Citations
  1. Ghait M, et al. 2022. Eur J Immunol. 52:907. PubMed
  2. Verigos J, et al. 2019. Cancers (Basel). :11. PubMed
  3. Kamperman T et al. 2019. Nat Commun. 10(1):4347 . PubMed
RRID
AB_315512 (BioLegend Cat. No. 508301)
AB_315512 (BioLegend Cat. No. 508302)

Antigen Details

Structure
Cytokine; 17.3 kD (Mammalian)
Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IL-1β Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human IL-1β JK1B-2 ELISA Detection,ELISPOT Detection
Purified anti-human IL-1β JK1B-2 ELISA
Go To Top Version: 3    Revision Date: 12/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human IL-1β

    Human_IL-1beta_TMB_020405

  • Purified anti-human IL-1β

    HumanIL-1beta_061604
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