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Biotin anti-mouse CD253 (TRAIL) Antibody

Pricing & Availability
Clone
N2B2 (See other available formats)
Regulatory Status
RUO
Other Names
TNFSF10, TNF-related apoptosis inducing ligand, Apo-2 ligand, Apo-2L, CD253
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
N2B2_Biotin_091907
Mouse TRAIL transfected cells stained with biotinylated N2B2, followed by Sav-PE
  • N2B2_Biotin_091907
    Mouse TRAIL transfected cells stained with biotinylated N2B2, followed by Sav-PE
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109303 50 µg £77
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Description

CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TRAIL-transfected 2PK-3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109308).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
  2. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  3. Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
  4. Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
  5. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
  6. Komatsu M, et al. 2003. Blood 101:3991. (Block)
  7. Taieb J, et al. 2006. Nature Med. 12:214. (Block)
  8. Gonzaga R, et al. 2011. J. Immunol. 187:6235. PubMed.
Product Citations
  1. Lee S, et al. 2013. Biochem Biophys Res Commun. 291:1999. PubMed
RRID
AB_345271 (BioLegend Cat. No. 109303)

Antigen Details

Structure
TNF ligand superfamily member, approximately 40 kD
Distribution

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Function
Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
Ligand/Receptor
TRAIL receptor DR5
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity. 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.

Gene ID
22035 View all products for this Gene ID
UniProt
View information about CD253 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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