Biotin anti-mouse Qa-2 Antibody anti-Qa-2

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Clone: 695H1-9-9 (See other available formats)
Regulatory Status: RUO
Other Names: Nonclassical MHC-Ib, H2-Qa2
Isotype: Mouse IgG2a, κ
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Product Citations: publications

695H1-9-9_Biotin_021808 — C57BL/6 mouse splenocytes stained with biotinylated 695H1-9-9, followed by Sav-PE

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121703

50 µg

£65

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121704

200 µg

£145

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Description

Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation².
RRID: AB_572000 (BioLegend Cat. No. 121703)
AB_572000 (BioLegend Cat. No. 121704)

Antigen Details

Structure: GPI-linked protein, associates with β2-microglobulin, 40 kD
Distribution: T and B cells, mature T cells expression higher than B cells
Function: Antigen presentation, promote immune response
Ligand/Receptor: Peptide
Cell Type: B cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Mann DW, et al. 1987. J. Immunol. 138:240.
3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920.
4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771.
5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367.
Gene ID: 111458 View all products for this Gene ID
UniProt: View information about Qa-2 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Qa-2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse Qa-2	695H1-9-9	FC
Alexa Fluor® 647 anti-mouse Qa-2	695H1-9-9	FC
FITC anti-mouse Qa-2	695H1-9-9	FC
Purified anti-mouse Qa-2	695H1-9-9	FC
APC anti-mouse Qa-2	695H1-9-9	FC
PE anti-mouse Qa-2	695H1-9-9	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 01/24/2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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