Loading...

Biotin anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
TER-119_Biotin_TER-119_Antibody_FC_041713
C57BL/6 bone marrow cells were stained with biotinylated TER-119 (filled histogram) or biotinylated rat IgG2b, κ isotype control (open histogram) and then detected with Sav-PE.
  • TER-119_Biotin_TER-119_Antibody_FC_041713
    C57BL/6 bone marrow cells were stained with biotinylated TER-119 (filled histogram) or biotinylated rat IgG2b, κ isotype control (open histogram) and then detected with Sav-PE.
Compare all formats
Cat # Size Price Quantity Check Availability Save
116203 50 µg £33
Check Availability


Need larger quantities of this item?
Request Bulk Quote
116204 500 µg £91
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  2. Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
  3. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  4. Cox BS, et al. 2022. JCI Insight. 7: . PubMed
  5. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  6. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  7. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  8. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  9. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  10. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  11. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  12. Theofilatos D, et al. 2022. STAR Protoc. 3:101839. PubMed
  13. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  14. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  15. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  16. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  17. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  18. Tang Y, et al. 2022. Dev Cell. 57:480. PubMed
  19. Gbyli R, et al. 2022. Leukemia. 36:1313. PubMed
  20. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  21. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  22. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  23. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  24. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  25. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  26. Riffelmacher T, et al. 2023. Nat Cell Biol. 25:877. PubMed
  27. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  28. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  29. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  30. Guo F, et al. 2022. Front Immunol. 13:833100. PubMed
  31. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  32. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  33. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  34. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  35. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  36. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  37. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  38. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  39. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  40. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  41. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  42. Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed
  43. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  44. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  45. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  46. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  47. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
  48. Hodzic D, et al. 2022. PLoS Biol. 20:e3001811. PubMed
  49. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  50. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  51. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  52. Iturri L, et al. 2021. Immunity. . PubMed
  53. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  54. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  55. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  56. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  57. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  58. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  59. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  60. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  61. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  62. Verena Sigl et al. 2017. Cell research. 26(7):761-74 . PubMed
  63. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  64. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  65. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  66. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  67. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  68. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  69. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  70. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  71. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  72. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  73. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  74. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  75. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  76. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  77. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  78. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  79. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  80. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  81. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  82. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  83. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  84. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  85. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  86. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  87. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  88. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  89. Peraki I, Palis J, and Mavrothalassitis G. 2017. Mol Cell Biol. 10.1128/MCB.00183-17. PubMed
  90. Greenwald AC, et al. 2019. J Exp Med. 216:215. PubMed
  91. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  92. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
  93. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  94. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  95. Dong S, et al. 2021. Nature. 591:117. PubMed
  96. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  97. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
  98. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  99. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  100. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  101. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  102. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  103. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  104. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  105. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  106. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  107. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  108. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  109. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  110. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  111. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  112. Wang C, et al. 2018. J Clin Invest. 128:3872. PubMed
  113. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  114. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  115. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  116. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  117. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  118. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  119. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  120. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  121. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  122. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  123. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  124. Gu VW, et al. 2021. Stem Cell Reports. 16:106. PubMed
  125. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  126. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  127. Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed
  128. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  129. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  130. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  131. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  132. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  133. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  134. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  135. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  136. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  137. Hasenberg M, et al. 2011. PLoS One. 6:e17314. PubMed
  138. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
  139. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  140. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  141. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  142. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  143. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  144. Koide S, et al. 2022. iScience. 25:103603. PubMed
  145. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  146. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  147. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  148. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
  149. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  150. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  151. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  152. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  153. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  154. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  155. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  156. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  157. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  158. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  159. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  160. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  161. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  162. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  163. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  164. Huang X, et al. 2021. Immunity. . PubMed
  165. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  166. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  167. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
  168. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  169. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  170. Young K, et al. 2021. Cell Stem Cell. . PubMed
  171. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  172. Seong J, et al. 2018. Development. 145:14. PubMed
  173. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
RRID
AB_313704 (BioLegend Cat. No. 116203)
AB_313704 (BioLegend Cat. No. 116204)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Go To Top Version: 3    Revision Date: 12/04/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account