Biotin anti-mouse TLR4 CD284/MD2 Complex Antibody anti-CD284/MD2

Pricing & Availability

Clone: MTS510 (See other available formats)
Regulatory Status: RUO
Other Names: Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Isotype: Rat IgG2a, κ
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Product Citations: publications

MTS510_Biotin_091907 — Thioglycollate-elicited mouse peritoneal macrophages were stained with biotinylated CD284 (clone MTS510) (filled histogram), or biotinylated rat IgG2a, κ (open histogram), followed by Sav- PE.

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117603

50 µg

£129

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Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.⁸ Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.⁸ In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
RRID: AB_313790 (BioLegend Cat. No. 117603)

Antigen Details

Structure: Type I transmembrane protein complex
Distribution: Monocytes, B cells and T cells
Function: Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor: Bacterial LPS
Cell Type: B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.
Gene ID: 21898 View all products for this Gene ID
UniProt: View information about TLR4 MD2 Complex on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All TLR4 (CD284)/MD2 Complex Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	FC,IHC-P,WB,ICFC,IP
Biotin anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	FC
PE anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	FC
PE/Cyanine7 anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	FC
Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	FC,IHC-P,ICFC,IP,Block
TotalSeq™-A0875 anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	PG
TotalSeq™-C0875 anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	PG
TotalSeq™-B0875 anti-mouse TLR4 (CD284)/MD2 Complex	MTS510	PG

Certificate of Analysis

Go To Top Version: 4 Revision Date: 11/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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