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Biotinylated Recombinant Human CD160 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD160 Antigen, Natural Killer Cell Receptor BY55, BY55, CD160Transmembrane Isoform, CD160-Delta Ig, NK28, NK1
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Product Citations
publications
BIOTIN_RECOM_Human_CD160_1_CF
Biotinylated recombinant human CD160 binds to immobilized recombinant human HVEM (Cat. No. 596504) in a dose-dependent manner using a functional ELISA. The binding was detected with HRP Avidin (Cat. No. 405103). The ED50 for this effect is 50 – 250 ng/mL.
  • BIOTIN_RECOM_Human_CD160_1_CF
    Biotinylated recombinant human CD160 binds to immobilized recombinant human HVEM (Cat. No. 596504) in a dose-dependent manner using a functional ELISA. The binding was detected with HRP Avidin (Cat. No. 405103). The ED50 for this effect is 50 – 250 ng/mL.
  • BIOTIN_RECOM_Human_CD160_2_CF
    Stability Testing for Biotinylated Recombinant Human CD160. Biotinylated Recombinant Human CD160 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to bind to immobilized recombinant human HVEM (Cat. No. 596504) in a dose-dependent manner. The binding was detected with HRP Avidin (Cat. No. 405103). The ED50 for this effect is 50 – 250 ng/mL.
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567004 25 µg £240
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567006 100 µg £414
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Description

CD160 is a glycosylphosphatidylinositol-attached membrane glycoprotein. It is a member of the Ig superfamily and possesses a single IgV-like domain. Four isoforms have been described for CD160 these include CD160-GPI, CD160 with a transmembrane domain (CD160-TM) and two more that do not have the IgV like domain (CD160DIg-GPI and CD160DIgTM). CD160 binds to MHC class I molecules with low affinity in humans and mice and enhances NK and CD8+ cytotoxic functions. In addition, CD160 binds to herpesvirus entry mediator (HVEM/TNFRSF14) with higher affinity than to MHC class I. The interaction between CD160 and HVEM inhibits T cell responses in vitro. In addition, HVEM binds to LIGHT/TNFSF14, LT-a, and BTLA (B and T lymphocyte attenuator). CD56dim NK cells show high expression of CD160 and their activation with HVEM, in the absence of other ligands, co-stimulates NK cell effector functions. In contrast, the interaction of HVEM to NK cells expressing CD160 and BTLA induces inhibition of effector functions. Studies with knockout mice showed that CD160 is essential for NK cells effector function in vivo and in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CD160, amino acid (Ile27-Ser159) (Accession: # O95971) with a C-terminus 6His and Avi-tag was expressed in CHO cells. Human CD160-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 169 amino acid recombinant protein has a predicted molecular mass of approximately 18.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 28 – 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.2 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick the spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human CD160 binds to immobilized recombinant human HVEM (Cat. No. 596504) in a dose-dependent manner using a functional ELISA. The binding was detected with HRP Avidin (Cat. No. 405103). The ED50 for this effect is 50 – 250 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

CD56dim CD16+ NK cells, NKT-cells, γδ T-cells, cytotoxic CD8+ T-cells, a small fraction of CD4+ T-cells, all intraepithelial lymphocytes, and epithelial cells

Function
CD160 binds to MHC class I molecules and enhances NK and T-cell cytolytic activity. CD160 interacts with HVEM (TNFRSF14) and inhibits the proliferation and the cytokine production of T cells.
Interaction
Antigen presenting cell, macrophages, DC, T and B cells
Ligand/Receptor
HLA-G1, HLA-C, and TNFRSF14
Bioactivity
Biotinylated recombinant human CD160 binds to immobilized recombinant human HVEM in a dose-dependent manner.
Cell Type
Endothelial cells, NK cells, NKT cells
Biology Area
Cell Proliferation and Viability, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling
Antigen References
  1. Mauri DN, et al. 1998. Immunity. 8:21-30.
  2. Barakonyi A, et al. 2004. J Immunol. 173:5349-54.
  3. Cai G, et al. 2008. Nat Immunol. 9:176-85.
  4. Giustiniani J, et al. 2009. J. Immunol. 182:63-71. 
  5. Šedý JR, et al. 2013. J Immunol. 191:828-36.
  6. El-Far M, et al. 2014. J Transl Med. 12:217.
  7. Tu TC, et al. 2015. J. Exp Med. 212:415-29.
  8. Muscate F, et al. 2018. Front Immunol. 9:2611.
Gene ID
11126 View all products for this Gene ID
UniProt
View information about CD160 on UniProt.org
Go To Top Version: 1    Revision Date: 01/25/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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