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Biotinylated Recombinant Human Mesothelin (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Antigen recognized by antigen K1 (CAK1, CAK1 antigen), megakaryocyte potentiating factor (MPF), soluble MPF, mesothelinreleated protein
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Product Citations
publications
Biotin_Human_Mesothelin_1_CF
Biotinylated recombinant human Mesothelin binds to immobilized CA125/MUC16 in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 0.27 – 1.35 µg/mL.
  • Biotin_Human_Mesothelin_1_CF
    Biotinylated recombinant human Mesothelin binds to immobilized CA125/MUC16 in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 0.27 – 1.35 µg/mL.
  • Biotin_Human_Mesothelin_2_CF
    Stability Testing for Biotinylated Recombinant Human Mesothelin. Biotinylated recombinant human Mesothelin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to bind to immobilized CA125/MUC16 in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 0.27 – 1.35 µg/mL.
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568404 25 µg £148
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568406 100 µg £476
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Description

Mesothelin (MSLN) was initially cloned from a library of human pancreatic cancer cells. It was also identified by a monoclonal antibody, MAb K1, which recognized a glycoprotein present on the surface of mesothelial cells. The human MSLN gene encodes a ~71 kD precursor protein of 622 amino acids. The C-terminal glycosylphosphatidylinositol (GPI) anchor addition signal is removed (cleavage site: Ser598) and the latter replaced with a GPI anchor. The MSLN precursor is cleaved by furin at Arg295 into two products, a ~31 kD mature megakaryocyte potentiating factor (MPF residues Ser34 - Arg286) and a ~40 kD GPI-anchored membrane-bound mature mesothelin starting from Glu296. The 40 kD GPI-anchored mesothelin can be released by treatment with phosphatidylinositol-specific phospholipase C. Soluble mesothelin specifically binds to ovarian carcinoma cell line OVCAR-3, mediated by the cancer antigen 125 (CA125), a mucin-like glycoprotein on the surface of tumor cells. CA125 and mesothelin are co-expressed in advanced grade ovarian adenocarcinoma, and it has been speculated that CA125 might contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to mesothelin. In addition, mesothelin and CA125/mucin 16 (CA125/MUC16) are co-overexpressed at the invading edges of pancreatic cancer tissues, and their expression correlates with poor survival rates. Mesothelin is highly expressed in several human cancers including malignant mesothelioma, pancreatic, ovarian, cholangiocarcinoma, and lung adenocarcinoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Biotinylated Recombinant Human Mesothelin, amino acid sequence (Glu296-Thr600) (Accession: # AAH09272.1), was expressed in 293E cells. The carboxy-terminus contains 6His-Avi tag. Human Mesothelin-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 341 amino acid recombinant protein has a predicted molecular mass of approximately 37.8 kD. The DTT-reduced and non-reduced proteins migrate at approximately 40 kD - 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Glu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human Mesothelin binds to immobilized CA125/MUC16 in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 0.27 – 1.35 µg/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

Tumor cells, mesothelial cells lining the pleura, peritoneum, and pericardium

Function
Cell adhesion. It may promote cancer cell survival, proliferation, and metastasis. It confers resistance to cytotoxic drug-induced apoptosis.
Interaction
Cancer cells
Ligand/Receptor
CA125/MUC16
Bioactivity
Biotinylated recombinant human Mesothelin binds to CA125/MUC16
Cell Type
Endothelial cells, Macrophages, NK cells, T cells
Biology Area
Cancer Biomarkers, Cell Adhesion
Molecular Family
Adhesion Molecules
Antigen References
  1. Kojima T, et al. 1995. J Biol Chem. 270:21984-90.
  2. Chang K and Pastan I. 1996. Proc Natl Acad Sci. USA. 93:136.
  3. Rump A, et al. 2004. J Biol Chem. 279:9190-8.
  4. Bharadwaj U, et al. 2011. Mol Cancer. 10:106.
  5. Chen SH, et al. 2013. Sci. Rep. 3:1870.
  6. Tang Z, et al. 2013. Anticancer Agents Med Chem. 13:276-80.
Gene ID
10232 View all products for this Gene ID
UniProt
View information about Mesothelin on UniProt.org
Go To Top Version: 1    Revision Date: 02/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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