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Biotinylated Recombinant Human TNFRSF17-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
BCM, BCMA, TNFRSF13A, CD269, B-cell maturation protein, B Cell Maturation Antigen, B-Cell Maturation Factor
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Product Citations
publications
Human_TNFRSF17_CF_RECOM_BA_1_112321
Biotinylated recombinant human TNFRSF17-Fc chimera binds to immobilized recombinant human April in a dose-dependent manner. The ED50 for this effect is 0.4 – 2.0 ng/mL.
  • Human_TNFRSF17_CF_RECOM_BA_1_112321
    Biotinylated recombinant human TNFRSF17-Fc chimera binds to immobilized recombinant human April in a dose-dependent manner. The ED50 for this effect is 0.4 – 2.0 ng/mL.
  • Human_TNFRSF17_CF_RECOM_BA_2_112321
    Stability Testing for Biotinylated Recombinant Human TNFRSF17-Fc Chimera. Biotinylated recombinant human TNFRSF17-Fc chimera was aliquoted in PBS, pH 7.2. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4⁰C (Control). The samples were tested for their ability to bind to immobilized recombinant human April in a dose-dependent manner. The ED50 for this effect is 0.4 – 2.0 ng/mL.
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796404 25 µg £197
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796406 100 µg £586
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Human TNFRSF17, a type I attached membrane protein of 184 amino acids, possesses a conserved motif of six cysteines in the N-terminal, consistent with the formation of a cysteine repeat motif found in the extracellular domain of TNFRs. Overexpression of TNFRSF17 in 293 cells activates NF-kappa B, Elk-1, the c-Jun N-terminal kinase, and the p38 mitogen-activated protein kinase, after its association with TNF Receptor-Associated Factor (TRAF) 1, TRAF2, and TRAF3. Soluble forms of TACI (TNFRSF13B) and TNFRSF17 bind to BAFF (TNFSFL13B) and APRIL (TNFSFL13A); the interaction of TNFRSF17 to APRIL has higher affinity than the TNFRSF17-BAFF interaction. Analysis of BCMA knockout mice (BCMA−/−) showed no differences in the steady-state frequencies or composition of peripheral B cell subsets (immature, marginal zone, follicular, B1). TNFRSF17 expression is upregulated on PBMCs from mice that develop a Sjogren’s-like syndrome, patients with Sjogren’s syndrome, with rheumatoid arthritis, and on B cells from SLE patients. Patients with multiple myeloma show elevated serum BCMA levels and it correlated with the proportion of plasma cells in bone marrow biopsies and clinical status. Serum BCMA levels is considered a novel biomarker to predict outcomes for multiple myeloma patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TNFRSF17, amino acids (Met1 – Ala54) (Accession # Q02223) with a linker (GS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in CHO cells. Human TNFRSF17-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 317 amino acid recombinant protein has a predicted molecular mass of approximately 35.0 KD. The DTT-reduced and non-reduced glycosylated protein migrate at approximately 45 kD and 80 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human TNFRSF17-Fc chimera binds to immobilized recombinant human April in a dose-dependent manner. The ED50 for this effect is 0.4 – 2.0 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Mature B lymphocytes, malignant B lymphocytes, plasma cells

Function
Promotes B-cell survival, B cell development
Interaction
Monocytes, macrophages, T cells, and dendritic cells
Ligand/Receptor
TNFSF13B/BLyS/BAFF and TNFSF13/APRIL
Bioactivity
Biotinylated recombinant human TNFRSF17-Fc chimera binds to immobilized recombinant human April.
Biology Area
Immunology
Molecular Family
Soluble Receptors
Antigen References
  1. Madry C, et al. 1998. Int Immunol. 10:1693-702.
  2. Hatzoglou A, et al. 2000. J Immunol. 165:1322-30.
  3. Rennert P, et al. 2000. J Exp Med. 192:1677-84.
  4. Wallweber HJ, et al. 2004. J Mol Biol. 2004 343:283-90.
  5. O'Connor BP, et al. 2004. J Exp Med. 199:91-8.
  6. Kim J, et al. 2011. Autoimmunity. 44:69-81.
  7. Coqueri CM, and Erickson LD. 2012. Crit Rev Immunol. 32:287-305.
  8. Laurent SA, et al. 2015. Nature Commun. 6:7333.
  9. Ghermezi M, et al. 2017. Hematologica. 102:785-795.
Gene ID
608 View all products for this Gene ID
UniProt
View information about TNFRSF17 on UniProt.org
Go To Top Version: 1    Revision Date: 11/23/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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