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Biotinylated Recombinant Human TNFRSF9 (4-1BB)-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD137, CDw137, 4-1BB, 4-1BB ligand receptor, T cell antigen ILA, T cell antigen 4-1BB homolog
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Product Citations
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Human_TNFRSF9_CF_BIOTIN_RECOM_1_040621.png
When recombinant human TNFSF9 (Cat. No. 750002) is immobilized at 1 μg/mL, biotinylated recombinant human TNFRSF9-Fc chimera binds in a dose-dependent manner with EC50 range of 0.75 – 3.0 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_TNFRSF9_CF_BIOTIN_RECOM_1_040621.png
    When recombinant human TNFSF9 (Cat. No. 750002) is immobilized at 1 μg/mL, biotinylated recombinant human TNFRSF9-Fc chimera binds in a dose-dependent manner with EC50 range of 0.75 – 3.0 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_TNFRSF9_CF_BIOTIN_RECOM_2_040621.png
    Stability Testing for Biotinylated Recombinant Human TNFRSF9-Fc Chimera. Biotinylated recombinant human TNFRSF9-Fc chimera was aliquoted in PBS, 5% glycerol at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with recombinant human TNFSF9.
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772614 25 µg £153
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772616 100 µg £362
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T-cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducers of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps optimal CD8 T-cell mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TNFRSF9, amino acid Leu24-Gln186 (Accession # Q07011.1), with a linker (SSLGS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in 293E cells. Human TNFRSF9-Fc Chimera was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 414 amino acid recombinant protein has a predicted molecular mass of approximately 45.46 kD. The DTT-reduced and non-reduced protein migrates at approximately 62 kD and 115 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, 5% Glycerol
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human TNFSF9 (Cat. No. 750002) is immobilized at 1 μg/mL, biotinylated recombinant human TNFRSF9-Fc chimera binds in a dose-dependent manner with EC50 range of 0.75 – 3.0 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide linked homodimer
Distribution

Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries

Function
Proliferation of T cells; activates their effector functions, survival, and establishes immunological memory.
Interaction
Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells
Ligand/Receptor
TNFSF9 and CD137L
Bioactivity
Measured by its ability to bind recombinant human TNFSF9.
Cell Type
B cells, Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immuno-Oncology, Immunology
Molecular Family
Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Pollok KE, et al. 1994. Eur J Immunol. 24:367-74.
  2. Watts TH. 2005. Annu Rev Immunol. 23:23-68.
  3. Zhu Y, et al. 2007. Blood. 109:4882-9.
  4. Locatelli F. 2009. Blood. 11:2208-9.
  5. Wang J, et al. 2010. J Immunol. 185:7654-62.
  6. Eun SY, et al. 2015. J Immunol. 1:134-41.
  7. Spencer AJ, et al. 2014. PLoS One. 9:e105520.
  8. Yi L, et al. 2014. PLoS One. 1:e86337
Gene ID
3604 View all products for this Gene ID
UniProt
View information about TNFRSF9 on UniProt.org
Go To Top Version: 1    Revision Date: 04/06/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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