- Clone
- H4B4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- LAMPb, LGP110
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
354307 | 25 µg | £125 | ||||
354308 | 100 µg | £268 |
CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines. LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Adult human adherent spleen cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of neutrophils2,3.
This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation. -
Application References
(PubMed link indicates BioLegend citation) -
- Chen J, et al. 1985. J. Biol. Chem. 101:85.
- Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
- Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
- RRID
-
AB_2721512 (BioLegend Cat. No. 354307)
AB_2721512 (BioLegend Cat. No. 354308)
Antigen Details
- Structure
- LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
- Distribution
-
Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines
- Function
- Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
- Cell Type
- B cells, Lymphocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538. - Gene ID
- 3920 View all products for this Gene ID
- UniProt
- View information about CD107b on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD107b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD107b (LAMP-2) | H4B4 | ICFC |
PE anti-human CD107b (LAMP-2) | H4B4 | ICFC |
Purified anti-human CD107b (LAMP-2) | H4B4 | ICFC,IHC-P,ICC,WB |
Alexa Fluor® 594 anti-human CD107b (LAMP-2) | H4B4 | IHC-P |
Alexa Fluor® 647 anti-human CD107b (LAMP-2) | H4B4 | ICFC,IHC-P |
Brilliant Violet 421™ anti-human CD107b (LAMP-2) | H4B4 | ICFC,IHC-P |
Brilliant Violet 785™ anti-human CD107b (LAMP-2) | H4B4 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... -
PE anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... -
Purified anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... Human paraffin-embedded prostate tissue slice was stained wi... ICC staining of purified anti-LAMP-2 antibody (clone H4B4) o... Western blot of purified anti-LAMP-2 antibody (clone H4B4). ... -
Alexa Fluor® 594 anti-human CD107b (LAMP-2)
Human paraffin-embedded prostate tissue slices were prepared... -
Alexa Fluor® 647 anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line KG1a was fixed, perme... Human paraffin-embedded prostate tissue slice were prepared ... -
Brilliant Violet 421™ anti-human CD107b (LAMP-2)
Human paraffin‐embedded prostate tissue slices were prepared... Human acute myeloid leukemia cell line KG1a was fixed, perme... -
Brilliant Violet 785™ anti-human CD107b (LAMP-2)
Human acute myeloid leukemia cell line, KG1a, was fixed, per...
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