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Brilliant Violet 421™ anti-human CD1c Antibody

Pricing & Availability
Clone
L161 (See other available formats)
Regulatory Status
RUO
Workshop
V T-CD01.18
Other Names
R7, M241, BDCA-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
L161_BV421_1_051311
Human peripheral blood lymphocytes were stained with CD19 APC and CD1c (clone L161) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom).
  • L161_BV421_1_051311
    Human peripheral blood lymphocytes were stained with CD19 APC and CD1c (clone L161) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom).
  • L161_BV421_2_051311
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
331525 25 tests £140
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331526 100 tests £275
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Description

CD1c, also known as R7 or M241, is a 43 kD member of the five CD1 antigens (CD1a-e) in humans. The CD1 molecules are type I glycoprotein with structural similarities to MHC class I and are non-covalently associated with β2-microglobulin, belonging to the Ig superfamily. CD1c is expressed on cortical thymocytes, Langerhans cells, dendritic cells, and a subset of B cells. It has been reported that CD1c is also expressed on mature T cells in a tightly regulated manner. CD1c is involved in antigen-presentation of glycolipids. It may also act in T cells as an immune regulatory molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue4, 5, formalin-fixed paraffin-embedded immunohistochemical staining6, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. del C Salamone M, et al. 2001. J Leukoc Biol. 70:567.
  2. de Fraissinette A, et al. 1988. Exp Hematol. 16:764.
  3. Li D, et al. 2012. J Exp Med. 209:109. PubMed
  4. Xu C, et al. 2010. Am J Hematol. 85:539 (IHC-F)
  5. Gerlini G, et al. 2001. J Invest Dermatol. 117:576 (IHC-F)
  6. Poposki J, et al. 2016. Clin Exp Allergy 45:384 (IHC-P) PubMed
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  3. Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed
  4. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  5. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  6. McCracken M, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006487. PubMed
  7. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  8. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  9. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  10. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  11. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  12. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  13. Gross C, et al. 2016. Proc Natl Acad Sci U S A. 113: 2973 - 2982. PubMed
  14. Ohmatsu H, et al. 2017. Oncoimmunology. 6:e1181237. PubMed
  15. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
  16. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  17. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  18. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_10962909 (BioLegend Cat. No. 331525)
AB_10962909 (BioLegend Cat. No. 331526)

Antigen Details

Structure
43 kD, Ig superfamily, MHC I-like molecule, associates with β2-microglobulin
Distribution

B cell subset, cortical thymocytes, dendritic cells, and Langerhans cells

Function
Presents lipid antigen to CD1c-restricted T cells
Ligand/Receptor
CD1c-restricted TCR
Cell Type
B cells, Dendritic cells, Langerhans cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Fainboim LM and del C. Salamone. 2002. J. Biol. Reg. Homeos. Ag. 16:125.
2. M. del Salamone C, et al. 2001. J. Leukocyte Biol. 70:567.
3. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. P42.

Gene ID
911 View all products for this Gene ID
UniProt
View information about CD1c on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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